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Plant regeneration via somatic embryogenesis in cotton

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Abstract

An efficient in vitro plant regeneration system characterized by rapid and continuous production of somatic embryos using leaf and stem explants of abnormal seedling as an explant have been developed in Gossypium hirsutum L. Embryogenic callus and somatic embryos have been obtained directly from the explants of cotton abnormal seedlings. Plant growth regulators influenced the induction of cotton somatic embryogenesis. The optimal medium for direct somatic embryogenesis was modified MS medium supplemented with 0.1 mg l-1 ZT and 2 g l-1 activated carbon. On this medium, an average of 28.0 and 28.1 matured somatic embryos formed from per leaf and stem explants respectively. The highest frequency of somatic embryogenesis was 100%. The somatic embryos were converted into normal plantlets when cultured on modified MS medium supplemented with 0.1 mg l-1 ZT. Upon transfer to soil, plants grew well and appeared normal. Plants could be regenerated within 60–80 days. The system of cotton somatic embryogenesis and plant regeneration described here will facilitate the application of plant tissue culture and genetic engineering on cotton genetic improvement.

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Author notes

  1. Bao-Hong Zhang

    Present address: , 1632 West 6th Street, APT F, Austin, TX 78703, USA

Authors and Affiliations

  1. Cotton Research Institute, Chinese Academy of Agricultural Sciences, Anyang Henan, 455112, P.R, China

    Bao-Hong Zhang, Fang Liu & Chang-Bing Yao

Authors
  1. Bao-Hong Zhang
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  2. Fang Liu
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  3. Chang-Bing Yao
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Correspondence to Bao-Hong Zhang.

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Zhang, BH., Liu, F. & Yao, CB. Plant regeneration via somatic embryogenesis in cotton. Plant Cell, Tissue and Organ Culture 60, 89–94 (2000). https://doi.org/10.1023/A:1006488119200

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  • DOI: https://doi.org/10.1023/A:1006488119200

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