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Regeneration of Indian ginseng plantlets from stem callus

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Abstract

The development of stem callus mediated plant regeneration system for Withania somnifera is described. Maximum callus proliferation was obtained on Murashige and Skoog medium supplemented with 2.26 μM 2,4-D. Three-week-old, white, friable callus was used for shoot regeneration. The maximum shoot regeneration (6.2 ± 0.34 shoots/explant) was achieved in four weeks when callus was cultured on MS medium fortified with 4.44 μM BA and 0.57 μM IAA. Regenerated shoots were excised and multiplied (8.4 ± 0.43 shoots/explant) on MS medium supplemented with 4.44 μM of BA. Multiple shoots were divided into single shoots and were rooted (5.1 ± 0.49 rootlets/shoot) on half strength MS medium supplemented with 9.84 μM of IBA. After a hardening phase of 3 weeks the plantlets were transferred to the field.

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Authors and Affiliations

  1. Centre for Biodiversity and Biotechnology, St. Xavier's College (Autonomous), Palayamkottai, 627 002, India

    V.S. Manickam, V.S. Manickam, R. Elango Mathavan & R. Antonisamy

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  1. V.S. Manickam
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  2. R. Elango Mathavan
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  3. R. Antonisamy
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Manickam, V., Elango Mathavan, R. & Antonisamy, R. Regeneration of Indian ginseng plantlets from stem callus. Plant Cell, Tissue and Organ Culture 62, 181–185 (2000). https://doi.org/10.1023/A:1006499522799

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