Abstract
Specific activities and mRNA levels of trypsin and amylase were studied in sea bass larvae. From day 20 to day 40, Dicentrarchus labrax were fed two rations of one day old Artemia: satiation (LP) and one-eighth of the satiation ration (LP/8) or two isoenergetic compound diets that varied in protein (30 and 60%) and carbohydrate (37 and 7%) content (FP30 and FP60 respectively). Trypsin mRNA levels and specific activities were mainly influenced by the nature of dietary protein and the Artemia ration. By using fish meal as protein source, dietary protein concentration did not affect either mRNA level nor specific activity of trypsin. These results suggested that the trypsin synthesis was not affected at a transcriptional level by the protein ration, i.e., Artemia ration. Decrease in amylase mRNA observed from day 29 in the four dietary groups suggested that this decrease in amylase expression is genetically programmed during sea bass larvae development. Nevertheless, the composition and the quantity of the diet influenced the amylase specific activities revealing primarily translational regulation of amylase. This study shows for the first time that the molecular mechanisms which control the dietary adaptation of trypsin and amylase are independently regulated, age-dependent and influenced by the composition and the quantity of the diet.
Similar content being viewed by others
References
Albertini-Berhaut, J. 1980. Biologie des stades juvéniles de mugilidae dans la région marseillaise: croissance, alimentation naturelle et activités enzymatiques. Thèse doctorale, Université Aix-Marseille, France.
Alliot, E. 1979. Enzymologie digestive. III. Evolution de quelques activités digestives au cours du développement larvaire des téléostéens. In Nutrition des poissons. Actes de colloque, pp. 79-87. Edited by M. Fontaine. CNERMA-Paris.
Beccaria, C., Diaz, J.P., Connes, R. and Chatain, B. (1991) Organogenesis of the exocrine pancraes in the sea bass, Dicentrarchus labrax L., reared extensively and intensively. Aquaculture. 99: 339-354.
Bradford, M.M. 1976. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal. Biochem. 72: 248-254.
Cahu, C.L. and Zambonino Infante, J.L. 1994. Early weaning of sea bass (Dicentrarchus labrax) larvae with a compound diet: effect on digestive enzymes. Comp. Biochem. Physiol. 109A: 213-222.
Corring, T. 1980. The adaptation of digestive enzymes to the diet: its physiological significance. Reprod. Nutr. Develop. 20: 1217-1235.
Corring, T., Juste, C. and Lhoste, E.F. 1989. Nutritional regulation of pancreatic and biliary secretions. Nutrition Reseach Reviews. 2: 161-180.
Duluc, I., Galluser, M., Raul, F. and Freund, J.N. 1992. Dietary control of lactase mRNA distribution along the rat small intestine. Am. J. Physiol. 262: G954-G961.
Dutrieu, J. 1960. Observations biochimiques et physiologiques sur le développement d'Artemia salina Leach. Archives de Zoologie Expérimentale et Générale. 99: 1-134.
Grendell, J.H. and Rothman, S.S. 1981. Digestive end products mobilize secretory proteins from subcellular stores in the pancreas. Am. J. Physiol. 241: G67-G73.
Henning, S.J. 1985. Ontogeny of enzymes in the small intestine. A. Rev. Physiol. 47: 231-345.
Henning, S.J. 1987. Functional development of the gastrointestinal tract. In Physiology of the Gastrointestinal Tract. pp. 285-300. 2nd edition. Edited by L.R. Johnson. Raven Press, New York.
Henning, S.J. 1994. Ontogeny of the intestinal mucosa. In Physiology of the Gastrointestinal Tract. pp. 571-610. 3rd edition. Edited by L.R. Johnson. Raven Press, New York.
Holm, H., Hanssen, L.E., Krogdahl, A. and Florholmen, J. 1988. High and low inhibitor soybean meals affect human duodenal proteinase activity differently: in vivo comparison with bovine serum albumin. J. Nutr. 118: 515-520.
Le Huërou, I., Wicker, C., Guilloteau, P., Toullec, R. and Puigserver, A. 1990. Specific regulation of the gene expression of some pancreatic enzymes during postnatal development and weaning in the calf. Biochim. Biophys. Acta. 1048: 247-264.
Le Huërou-Luron, I., Lhoste, E., Wicker-Planquart, C., Dakka, N., Toullec, R., Corring, T., Guilloteau, P. and Puigserver, A. 1993. Molecular aspects synthesis in the exocrine pancreas with emphasis on development and nutritional regulation. Proceeding of the Nutrition Society. 52: 301-313.
Lhoste, E.F., Fiszlewicz, M., Gueugneau, A.M. and Corring, T. 1994. Adaptation of exocrine pancreas to dietary proteins: Effect of the nature of protein and rat strain on enzyme activities and messager RNA levels. J. Nutr. Biochem. 5: 84-94.
McLaughlin, R.L., Ferguson, M.M. and Noakes, L.G. 1995. Tissues concentrations of RNA and protein for juvenile brook trout (Salvelinus fontilalis): lagged responses to fluctuations in food availability. Fish Physiol. Biochem. 14: 459-469.
Métais, P. and Bieth, J. 1968. Détermination de l'α-amylase par une microtechnique. Ann. Biol. Clin. 26: 133-142.
Péres, A., Cahu, C.L., Zambonino Infante, J.L., Le Gall, M.M. and Quazuguel, P. 1996. Amylase and trypsin responses to intake of dietary carbohydrate and protein depend on the development stage in sea bass (Dicentrarchus labrax) larvae. Fish Physiol. Biochem. 15: 237-242.
Person-Le Ruyet, J., Alexandre, J.C., Thébaud, L. and Mugnier, C. 1993. Marine fish larvae feeding: formulated diets or live preys? J. World Aquac. Soc., 24: 211-224.
Richard, P., Bergeron, J.P., Boulhic, M., Galois, R. and Person-Le Ruyet, J. 1991. Effect of starvation on RNA, DNA and protein content of laboratory-reared larvae and juveniles of Solea solea. Mar. Ecol. Prog. Ser. 72: 69-77.
Rossi, T.M., Lee, P.C., Young, C.M., Lerner, A. and Lebenthal, E. 1986. Effect of nutritional rehabilitation of the development of intestinal brush border disaccharidases of postnatal malnourished weanling rats. Pediatr. Res. 20: 793-797.
Scheele, G.A. 1993. Regulation of pancreatic gene expression in response to hormones and nutritional substrates. In The Pancreas: Biology, Pathobiology, and Disease. pp. 103-120. 2nd edition. Edited by V.L.W Go, F.P. Gardner, F.P. Brooks, E. Lebenthal, E.P. Di Magno and G.A. Scheele. Raven Press, New York.
Smith, L.S. 1989. Digestive functions in teleost fishes. In Fish Nutrition. pp. 331-422. Edited by J.E. Halver. Academic press, New York.
Sölch, J.P. and Arnold, G.J. 1996. Multiplex reverse transcription polymerase chain reaction combined with temperature gradient gel electrophoresis as a tool for the normalized quantitation of intrinsic factor mRNA. Electrophoresis. 17: 30-39.
Steinhilber W., Poensgen J., Rausch, U., Kern, H.F. and Scheele, G. 1988. Translational control of anionic trypsinogen and amylase synthesis in response to caerulein stimulation. Proc. Natl. Acad. Sci. USA. 85: 6597-6601.
Tseng, H.C., Grendell, J.H. and Rothman, S.S. 1982. Food, duodenal extracts, and enzyme secretion by the pancreas. Am. J. Physiol. 243: G304-G312.
Vu, T.T. 1983. Etude histoenzymologiques des activités protéasiques dans le tube digestif des larves et des adultes de bar, Dicentrarchus labrax. Aquaculture. 32: 57-69.
Walford, J. and Lam, T.J. 1991. Development of digestive tract and proteolitic enzyme activity in sea bass (Lates calcarifer) larvae and juveniles. Aquaculture. 109: 187-205.
Wicker, C., Puigserver, A., Rausc, U., Scheele, G. and Kern, H. 1985. Multiple-level caerulein control of the gene expression of secretory proteins in the rat pancreas. Eur. J. Biochem. 151: 461-466.
Zambonino Infante, J.L. and Cahu, C. 1994. Development and response to a diet change of some digestive enzymes in sea bass. Fish Physiol. Biochem. 12: 399-408.
Zambonino-Infante, J.L., Cahu, C.L., Péres, A., Quazuguel, P. and Le Gall, M.M. 1996. Sea bass (Dicentrarchus labrax) larvae fed different Artemia rations: growth, pancreas enzymatic response and development of digestive functions. Aquaculture 139: 129-138.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Péres, A., Zambonino Infante, J. & Cahu, C. Dietary regulation of activities and mRNA levels of trypsin and amylase in sea bass (Dicentrarchus labrax) larvae. Fish Physiology and Biochemistry 19, 145–152 (1998). https://doi.org/10.1023/A:1007775501340
Issue Date:
DOI: https://doi.org/10.1023/A:1007775501340