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Sequence Analysis Demonstrates that VP6, NSP1 and NSP4 Genes of Indian Neonatal Rotavirus Strain 116E are of Human Origin

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Abstract

We have sequenced the genes encoding the inner capsid protein VP6 and the nonstructural proteins NSP1 and NSP4 of the Indian neonatal serotype P8[11]G9 human/bovine reassortant candidate vaccine rotavirus strain 116E. These three genes share a high degree of sequence and deduced amino acid homology with human prototype strain Wa. Our results confirm and extend those of previous RNA-RNA hybridization studies which suggested that these genes are of human origin, and will facilitate examination of the host immune response to 116E induced by natural infection and vaccination.

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Authors and Affiliations

  1. Division of Viral and Rickettsial Diseases, National Center for Infectious Diseases, Centers for Disease Control and Prevention, Department of Health and Human Services, Viral Gastroenteritis Section, Atlanta, Georgia

    Nigel A. Cunliffe, Roger I. Glass & Jon R. Gentsch

  2. Department of Medical Microbiology and Genito-urinary Medicine and Wellcome Trust Liverpool Centre for Clinical Tropical Medicine, University of Liverpool, Liverpool, UK

    Nigel A. Cunliffe

  3. Departments of Pediatrics and Microbiology, All India Institute of Medical Sciences, New Delhi, India

    Bimal K. Das, Madhumati Ramachandran & Maharaj K. Bhan

Authors
  1. Nigel A. Cunliffe
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  2. Bimal K. Das
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  3. Madhumati Ramachandran
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  4. Maharaj K. Bhan
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  5. Roger I. Glass
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  6. Jon R. Gentsch
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Cunliffe, N.A., Das, B.K., Ramachandran, M. et al. Sequence Analysis Demonstrates that VP6, NSP1 and NSP4 Genes of Indian Neonatal Rotavirus Strain 116E are of Human Origin. Virus Genes 15, 39–44 (1997). https://doi.org/10.1023/A:1007958914141

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  • DOI: https://doi.org/10.1023/A:1007958914141

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