Abstract
The efficacy of Trichoderma harzianum T39 and the yeasts Aureobasidium pullulans and Cryptococcus albidus against Botrytis cinerea in cucumber and tomato was compared with chemical control. Four experiments were conducted in cucumber grown under different climatic conditions in The Netherlands, and two experiments were done in tomato both in the Netherlands and in Israel. T. harzianum and A. pullulans showed the most consistent control of B. cinerea, reducing stem lesions and death of plants by 40–100% in most cases. Control of stem lesions and subsequent wilting was generally better than control of symptoms on fruits. In some cases, the biocontrol agents were more effective than the broad-spectrum fungicide tolylfluanid and the selective fungicide iprodione. The climatic conditions did not strongly influence the efficacy of the biocontrol agents, but regression analysis showed that high temperature during the day and high vapour pressure deficit during the night reduced biocontrol efficacy. From the results, prospects for biocontrol of B. cinerea in greenhouse vegetables appear good under a range of conditions.
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References
Bakker JC (1991) Analysis of humidity effects on growth and production of glasshouse fruit vegetables. Dissertation, Wageningen Agricultural University, Wageningen, The Netherlands, 155 pp
Campbell CL and Madden LV (1990) Introduction to plant disease epidemiology. John Wiley and Sons, Inc., New York, NY, USA. 532 pp
De Meyer G, Bigirimana J, Elad Y and Hofte M (1998) Induced systemic resistance in Trichoderma harzianum T39 biocontrol of Botrytis cinerea. Eur J Plant Pathol 104: 279-280
Dik AJ and Buitelaar K (1995) Voorlopig niet snijden maar breken of knippen. Groente en Fruit/Glasgroenten 3: 14-15
Dik AJ, Koning G and Köhl J (1999) Evaluation of microbial antagonists for biological control of Botrytis cinerea stem infection in cucumber and tomato. Eur J Plant Pathol 105: 115-122
Elad Y and Kirshner B (1993) Survival in the phylloplane of an introduced biocontrol agent (Trichoderma harzianum) and populations of the plant pathogen Botrytis cinerea as modified by abiotic conditions. Phytoparasitica 21: 303-313
Elad Y, Malathrakis NE and Dik AJ (1996) Biological control of Botrytis-incited diseases and powdery mildews in greenhouse crops. Crop Prot 15: 229-240
Elad Y, Yunis H and Katan J (1992) Multiple resistance to benzimidazoles, dicarboximides and diethofencarb in field isolates of Botrytis cinerea in Israel. Plant Pathology 41: 41-46
Elad Y, Zimand G, Zaqs Y, Zuriel S and Chet Y (1993) Biological and integrated control of cucumber grey mould (Botrytis cinerea) under commercial greenhouse conditions. Plant Pathology 42: 324-332
Flood J and Rees J (1986) Host produced toxins associated with antagonism by Aureobasidium pullulans against Alternaria solani on wounded tomato leaves. Physiol Mol Plant Pathol 28: 79-88
Fry WE (1978) Quantification of general resistance of potato cultivars and fungicide effects for integrated control of potato late blight. Phytopathology 68: 1650-1655
Genstat 5 Committee (1992) Genstat 5 Release 3 Reference Manual. Oxford University Press Inc, Oxford, UK. 796 pp
Graaf R de (1985) The influence of thermal screening and moisture gap on the transpiration of glasshouse tomatoes during the night. Acta Horticulturae 174: 57-63
Jarvis WR (1992) Managing Diseases in Greenhouse Crops. APS Press, Minneapolis, MN, USA. 288 pp
Koning GP and Köhl J (1995) Wound protection by antagonists against Botrytis stem rot in cucumber and tomato. In: M. Manka (ed.) Environmental Biotic Factors in Integrated Plant Disease Control, 313-316, The Polish Phytopathological Society, Poznan
O'Neill TM, Niv A, Elad Y and Shtienberg D (1996) Biological control of Botrytis cinerea on tomato stem wounds with Trichoderma harzianum. Eur J Plant Pathol 102: 635-643
Schrattenholz A and Flesch P (1993) Isolation, structural and toxicological characterization of three new mycotoxins produced by the fungus Aureobasidium pullulans. Mycotoxin Res 9: 13-21
Shtienberg D and Elad Y (1997) Incorporation of weather forecasting in integrated, biological-chemical management of Botrytis cinerea. Phytopathology 87: 332-340
Steekelenburg NAM van (1987) Resistance to benzimidazole and dicarboximide fungicides in Botrytis cinerea and Dydimella bryoniae in cucumbers in the Netherlands. Med Fac Landbouww. Rijksuniv. Gent 52(3a): 875-880
Yunis H, Elad Y Mahrer Y (1990) Effects of air temperature, relative humidity and canopy wetness on grey mould of cucumbers in unheated greenhouses. Phytoparasitica 18: 203-215
Winspear KW, Postlethwaite JD and Cotton RF (1970) The restriction of Cladosporium fulvum and Botrytis cinerea, attacking greenhouse tomatoes, by automatic humidity control. Ann of Appl Biol 65: 75-83
Zimand G, Elad Y and Chet I (1996) Effect of Trichoderma harzianum on Botrytis cinerea pathogenicity. Phytopathology 86: 1255-1260
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Dik, A., Elad, Y. Comparison of Antagonists of Botrytis cinerea in Greenhouse-grown Cucumber and Tomato under Different Climatic Conditions. European Journal of Plant Pathology 105, 123–137 (1999). https://doi.org/10.1023/A:1008778213278
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DOI: https://doi.org/10.1023/A:1008778213278