Skip to main content
SpringerLink
Log in

Rapid method for the determination of lipid from the green alga Botryococcus braunii

  • Published:
Biotechnology Techniques

Abstract

Of various methods for lipid recovery in Botryococcus braunii UTEX 572, the most effective method was disruption of the cells with a bead-beater followed by extraction with chloroform/methanol (2:1, v/v). This gave a lipid content of 28.6% of dry wt. There was a significant relationship between in vivo fluorescence of cells stained with Nile Red and lipid content in B. braunii determined gravimetrically (r2 = 0.997). This suggested that the Nile Red staining as a rapid method was as good as the gravimetric method commonly used for lipid determination which requires toxic solvents and considerable time-consuming manipulations. © Rapid Science Ltd. 1998

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Ahlgren, G and Uppsala, LM (1991). Arch Hydrobiol 121:295-306

    Google Scholar 

  • Bligh, EG and Dyer, WJ (1959). Can J Biochem Physiol 37:911-917

    Google Scholar 

  • Casadevall, E, Dif, D, Largeau, C, Gudin, C, Chaumont, D and Desant, O (1985). Biotechnol Bioeng 27:286-295

    Google Scholar 

  • Cooksey, KE, Guckert, JB, Williams, SA and Callis, PR (1987). J Microbiol Meth 6:333-345

    Google Scholar 

  • Damyanova, BN, Stefanov, K, Seizova, K and Popov, S (1992). Comp Biochem Phys 103B:733-736

    Google Scholar 

  • Greenspan, P, Mayer, EP and Fowler SD (1985). J Cell Biol 100:965-973

    Google Scholar 

  • Guckert, JB, Cooksey, KE and Jackson, LL (1988). J Microbiol Meth 8:139-149

    Google Scholar 

  • Hara, A and Radin, NS (1978). Anal Biochem 90:420-426

    Google Scholar 

  • Lee, SJ, Kim, S-B, Kim, J-E, Kwon, G-S, Yoon, B-D and Oh, H-M (1998). Lett Appl Microbiol 26:(in press)

  • McGinnis, KM, Dempster, TA and Sommerfeld, MR (1997). J Appl Phycol 9:19–24

    Google Scholar 

  • Ryan, TW, Doge, LG and Callahan, TJ (1984). J Am Oil Chem Soc 61:1610-1619

    Google Scholar 

  • Sawayama, S, Inoue, S and Yokoyama, S (1994). Appl Microbiol Biot 41:729–731

    Google Scholar 

  • Yamaguchi, M, Nakano, H, Murakami, M, Konosu, S, Nakayama, O, Kanda, M, Nakamura, A and Iwamoto, H (1987). Agric Biol Chem 51:493-498

    Google Scholar 

Download references

Authors
  1. Seog June Lee
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Byung-Dae Yoon
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Hee-Mock Oh
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Lee, S.J., Yoon, BD. & Oh, HM. Rapid method for the determination of lipid from the green alga Botryococcus braunii. Biotechnology Techniques 12, 553–556 (1998). https://doi.org/10.1023/A:1008811716448

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/A:1008811716448

Keywords

Search

Navigation