Abstract
The evaluation of soil lipase activity as a tool to monitor the decontamination of a freshly oil-polluted soil was tested in a laboratory study. An arable soil was experimentally contaminated with diesel oil at 5 mg hydrocarbons g−1 soil dry weight and incubated with and without fertilization (N-P-K) for 116 days at 20 °C. Lipase activity and counts of oil-degrading microorganisms were measured at regular time intervals, and the correlations with the levels of hydrocarbon concentrations in soil were investigated. The residual soil hydrocarbon concentration correlated significantly negatively with soil lipase activity and with the number of oil-degrading microorganisms, independent of fertilization. The induction of soil lipase activity is a valuable indicator of oil biodegradation in naturally attenuated (unfertilized) and bioremediated (fertilized) soils.
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References
Allard AS, Neilson AH (1997) Int. Biodeterioration Biodegradation 39: 253-285.
Bayer H, Mitterer M, Schinner F (1982) Pedobiologia 23: 311-319.
Brunner I, Schinner F (1994) Die Bodenkultur 35: 1-12.
Dick RP (1997) Soil enzyme activities as integrative indicators of soil health. In: Pankhurst CE, Double, BM, Gupta VV, eds. Biological Indicators of Soil Health. Oxon: CAB International, pp. 121-157.
DIN 38409-H18 (1981) Deutsche Einheitsverfahren zur Wasser-, Abwasser-und Schlammuntersuchung. Bestimmung von Kohlenwasserstoffen (H18). Berlin: Deutsches Institut für Normung eV.
Frankenberger WT, Johanson JB (1982) J. Environ. Qual. 11: 602-607.
Hinchee RE (1998) In situ bioremediation: practices and challenges. In: Serra R, ed. Biotechnology for Soil Remediation. Milan: CIPA, pp. 17-20.
Janke S, Schamber H, Kunze C (1992) Angew. Bot. 66: 42-45.
Margesin R, Schinner F (1997) Appl. Microbiol. Biotechnol. 47: 462-468.
Margesin R, Schinner F (1999a) World J. Microbiol. Biotechnol. 15: in press.
Margesin R, Schinner F (1999b) J. Chem. Technol. Biotechnol. 74: 1-9.
Norris RD (ed.) (1994) Handbook of Bioremediation. Boca Raton, FL: CRC Press.
Pokorna V (1964). Soviet Soil Sci. 1: 85-87.
Schinner F, Öhlinger R, Kandeler E, Margesin R (eds) (1996) Methods in Soil Biology. Berlin: Springer-Verlag.
van Beelen P, Doelman P (1997) Chemosphere 34: 455-499.
van der Waarde JJ, Dijkhuis EJ, Henssen MJC, Keuning S (1995) In: Hinchee RE, Douglas GS, Ong SK, eds. Monitoring and Verification of Bioremediation. Columbus: Battelle Press, pp. 59-63.
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Margesin, R., Zimmerbauer, A. & Schinner, F. Soil lipase activity – a useful indicator of oil biodegradation. Biotechnology Techniques 13, 859–863 (1999). https://doi.org/10.1023/A:1008928308695
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DOI: https://doi.org/10.1023/A:1008928308695