Abstract
Two peptides corresponding to putative protective regions located at the N- and C-termini of the host-protective T. ovis recombinant antigen, 45W, were synthesized. Antibodies raised against 45W and 45WB/X, a truncated form of 45W, were found to react strongly with the N-terminal peptide. When sheep were immunised with each peptide alone, the N-terminal peptide was found to be highly immunogenic, whereas the C-terminal peptide required conjugation to a carrier protein to be immunogenic. Both these immunogens elicited antibodies that cross-reacted with the parent protein; however, only antibodies directed toward the N-terminal peptide were able to bind antigens from the T. ovis oncosphere. Significant protection against challenge infection was not provided by any of the peptide immunogens used.
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References
Anderer, F., Biochim. Biophys. Acta, 71 (1963) 246.
Ben-Yedidia, T. and Arnon, R., Curr. Opin. Biotech., 8 (1997) 442.
Berzofsky, J.A., Semin. Immunol., 3 (1991) 203.
Milich, D.R., Semin. Immunol., 2 (1990) 307.
Belyakov, I.M., Derby, M.A., Ahlers, J.D., Kelsall, B.L., Earl, P., Moss, B., Strober, W. and Berzofsky, J.A., Proc. Natl. Acad. Sci. USA, 95 (1998) 1709.
Bharadwaj, A., Sharma, P., Joshi, S.K., Singh, B. and Chauhan, V.S., Infect. Immun., 66 (1998) 3232.
Genco, C.A., Odusanya, B.M., Potempa, J., Mikolajczyk-Pawlinska, J. and Travis, J., Infect. Immun., 66 (1998) 4108.
Ghosh, S. and Jackson, D.C., Int. Immunol., 11 (1999) 1103.
Schutze, M.P., Leclerc, C., Jolivet, M., Audibert, F. and Chedid, L., J. Immunol., 135 (1985) 2319.
Jacob, C.O., Arnon, R. and Sela, M., Mol. Immunol., 22 (1985) 1333.
Herzenberg, L.A., Tokuhisa, T. and Herzenberg, L.A., Nature, 285 (1980) 664.
Dyrberg, T. and Oldstone, M.B., J. Exp. Med., 164 (1986) 1344.
Briand, J.P., Muller, S. and van Regenmortel, M.H.V., J. Immunol. Methods, 78 (1985) 59.
Johnson, K.S., Harrison, G.B., Lightowlers, M.W., O'Hoy, K.L., Cougle, W.G., Dempster, R.P., Lawrence, S.B., Vinton, J.G., Heath, D.D. and Rickard, M.D., Nature, 338 (1989) 585.
Rickard, M.D., Harrison, G.B., Heath, D.D. and Lightowlers, M.W., Parasitology, 110 (1995) S5.
Rothel, J.S., Lightowlers, M.W., Seow, H.F., Wood, P.R., Rothel, L.J., Heath, D.D. and Harrison, G.B., Parasite Immunol., 18 (1996) 201.
Heath, D.D., Holcman, B. and Shaw, R.J., Int. J. Parasitol., 24 (1994) 929.
Rogan, M.T., Craig, P.S., Zehyle, E., Masinde, G., Wen, H. and Zhou, P., Acta Trop., 51 (1992) 291.
Heath, D.D. and Lawrence, S.B., Int. J. Parasitol., 11 (1981) 261.
Heath, D.D. and Pavloff, P., Int. J. Parasitol., 5 (1975) 83.
Rickard, M.D. and Outteridge, P.M., Z. Parasitenkd, 44 (1974) 187.
Waterkeyn, J.G., Lightowlers, M.W., Coppel, R. and Cowman, A.F., Mol. Biochem. Parasitol., 73 (1995) 123.
Lightowlers, M.W., Waterkeyn, J.G., Rothel, J.S., Gauci, C.G. and Harrison, G.B., Parasite Immunol., 18 (1996) 507.
Dempster, R.P., Robinson, C.M. and Harrison, G.B., Parasitol. Res., 82 (1996) 291.
Harrison, G.B., Heath, D.D., Dempster, R.P., Lawrence, S.B., Lightowlers, M.W. and Rickard, M.D., Int. J. Parasitol., 23 (1993) 41.
Laemmli, U.K., Nature, 227 (1970) 680.
Towbin, H., Staehelin, T. and Gordon, J., Proc. Natl. Acad. Sci. USA, 76 (1979) 4350.
Jameson, B.A. and Wolf, H., Comput. Appl. Biosci., 4 (1988) 181.
Emini, E.A., Hughes, J.V., Perlow, D.S. and Boger, J., J. Virol., 55 (1985) 836.
Karplus, P.A. and Schultz, G.E., Naturwissenschaften, 72 (1985) 212.
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Dadley-Moore, D.L., Lightowlers, M.W., Gauci, C.G. et al. Synthetic peptides induce antibodies in sheep against Taenia ovis. Letters in Peptide Science 6, 303–312 (1999). https://doi.org/10.1023/A:1008942132205
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DOI: https://doi.org/10.1023/A:1008942132205