Abstract
Chlorophenol degradation was studied by combined anaerobic–aerobic treatments as a single or multi-substrate system. 2,4-Dichlorophenol (2,4-DCP) was degraded to the extent of 52 and 78% in up-flow anaerobic sludge blanket (UASB) and aerobic suspended growth (ASG) reactors respectively, at organic loading rates of 0.18 kg/m3/day and hydraulic retention time of 26.4 h in the presence of glucose. The UASB represents the dominating facultative anaerobic microbial population. When the effluent from the anaerobic reactor (UASB) was subjected to aerobic treatment on the ASG reactor, 2,4-DCP and COD removals of 86 and 95% respectively were achieved. Aerobic degradation of chlorophenol by acclimated mixed bacterial isolates was found to be sequential: 2-Chlorophenol (2-CP) and 4-CP were degraded first, followed by 2,4-DCP and 2,4,6-Trichlorophenol (2,4,6-TCP) while the contrary was obtained in anaerobic degradation. In anaerobic degradation by acclimated mixed bacterial cells, 2,4-DCP and 2,4,6-TCP were degraded first followed by mono-chlorophenols. The anaerobic/aerobic bioreactors were most efficient when operated in sequence (series) rather than in parallel.
References
APHA, AWWA, WPCF 1995 Standard Methods for Examination of Water and Waste-water. American Public Health Association 19th edn. Washington DC ISBN 0-87553223-3.
Ataberk, S. & Gokcay, C.F. 1997 Treatment of chlorinated organics in bleached Kraft effluent by activated sludge. Water Science and Technology 36(2/3), 147-150.
Bhaumik, S., Christodoulatos, C., Korfigtis, G.P. & Brodman, B.W. 1997 Aerobic and anaerobic biodegradation of nitroglycerin in batch and packed bed reactors. Water Science and Technology 36, 139-146.
Bultron, G., Gonzalez, A. & Lopez-Marin, L.M. 1998 Biodegradation of phenolic compounds by an acclimated activated sludge and isolated bacteria. Water Science and Technology 37, 371-378.
Chakrabarti, T., Subrahmanyam, P.V.R. & Sundaresan, B.B. 1988 Biodegradation of recalcitrant industrial wastes. In Biotreatment Systems. Vol. 2, ed. Wise, D.L. Florida: CRC Press Inc. ISBN 0-84934849-8.
Chatterjee, D.K., Kellog, S.T., Hamda, S. & Chakarbarti, A.M. 1981 Plasmid specifying total degradation of 3-chlorbenzoate by a modified ortho-pathway. Journal of Bacteriology 146, 639-646.
Christiansen, N., Hendriksen, H.V., Jurvinen, K.T. & Ahring, B.K. 1995 Degradation of chlorinated aromatic compounds in UASB reactors. Water Science and Technology 31, 2249-2259.
Ferguson, J.F. 1994 Anaerobic and aerobic treatment for AOX removal. Water Science and Technology 29, 149-162.
Knackmuss, H.J. & Reineke, W. 1988 Microbial degradation of haloartomatics. Annual Review of Microbiology 42, 263-287.
Latkar, M. & Chakrabarti, T. 1994 Resorcinol, catechol and Hydroquinone biodegradation in mono and binary substrate matrices in up-flow anaerobic fixed-film fixed-bed reactors. Water Research 26, 599-607.
Sheldon, J.B., Kenedy, J.K. & Brady, J.A. 1995 Treatment of phenol/ PCP wastewater using UASB reactor. Water Research 29, 645-651.
Sulfita, J.M. & Concannon, F. 1995 Screening tests for assessing the anaerobic biodegradation of pollutant; Chemicals in subsurface environment. Journal of Microbiological Methods 21, 267-281.
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Atuanya, E., Purohit, H. & Chakrabarti, T. Anaerobic and aerobic biodegradation of chlorophenols using UASB and ASG bioreactors. World Journal of Microbiology and Biotechnology 16, 95–98 (2000). https://doi.org/10.1023/A:1008957229070
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DOI: https://doi.org/10.1023/A:1008957229070