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Electrochemical activity of an Fe(III)-reducing bacterium, Shewanella putrefaciens IR-1, in the presence of alternative electron acceptors

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Biotechnology Techniques

Abstract

Cyclic voltammetry demonstrated that cells of Shewanella putrefaciens grown under anaerobic conditions without nitrate were electrochemically active. The electrochemical activity was inactivated reversibly by exposure to air, but not by nitrate. Lactate and an applied potential at +200 mV against an Ag/AgCl reference electrode restored the electrochemical activity. These findings can be used to improve the performance of a mediator-less microbial fuel cell using electrochemically active bacteria in the presence of nitrate.

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References

  • Arnold RG, DiChristina TJ, Hoffmann MR (1986) Inhibitor studies of dissimilatory Fe(III) reduction by Pseudomonas sp. Strain 200 ('Pseudomonas ferrireductans'). Appl. Environ. Microbiol. 52: 281-289.

    Google Scholar 

  • DiChristina TJ (1992) Effects of nitrate and nitrite on dissimilatory iron reduction by Shewanella putrefaciens 200. J. Bacteriol. 174: 1891-1896.

    Google Scholar 

  • DiChristina TJ, DeLong EF (1994) Isolation of anaerobic respiratory mutants of Shewanella putrefaciens and genetic analysis of mutants deficient in anaerobic growth on Fe3+. J. Bacteriol. 176: 1468-1474.

    Google Scholar 

  • Guest JR (1992) Oxygen-regulated gene expression in Escherichia coli. J. Gen. Microbiol. 138: 2253-2263.

    Google Scholar 

  • Higgins IJ, Hill HAO (1985) Bioelectrochemistry. Essays Biochem. 21: 119-145.

    Google Scholar 

  • Kazlauskaite J, Hill HAO, Wilkins PC, Dalton H (1996) Direct electrochemistry of the hydroxylase of soluble methane monooxygenase from Methylococcus capsulatus (Bath). Eur. J. Biochem. 241: 552-556.

    Google Scholar 

  • Kim TS, Kim BH (1988) Modulation of Clostridium acetobutylicum fermentation by electrochemically supplied reducing equivalent. Biotechnol. Lett. 10: 123-128.

    Google Scholar 

  • Kim TS, Kim HY, Kim BH (1990) Petroleum desulfurization by Desulfovibrio desulfuricans M6 using electrochemically supplied reducing equivalent. Biotechnol. Lett. 12: 757-760.

    Google Scholar 

  • Kim HJ, Kim BH, Hyun MS, Cho BW (1998) Performance of a mediator-less microbial fuel cell using metal reducing bacterium, Shewanella putrefaciens IR-1. Abstr. 98th Am. Soc. Microbiol. Washington, D.C.: American Society for Microbiology, p. 395.

    Google Scholar 

  • Kim BH, Kim HJ, Hyun MS, Park DH (1999) Direct electrode reaction of Fe(III)-reducing bacterium, Shewanella putrefaciens. J. Microbiol. Biotechnol. 9: 127-131.

    Google Scholar 

  • Myers CR, Myers JM (1992) Localization of cytochromes to the outer membranes of anaerobically grown Shewanellaputrefaciens MR-1. J. Bacteriol. 174: 3429-3438.

    Google Scholar 

  • Nealson KH, Saffarini D (1994) Iron and manganese in anaerobic respiration: Environmental significance, physiology, and regulation. Annu. Rev. Microbiol. 48: 311-343.

    Google Scholar 

  • Park DH, Kim BH, Moore B, Hill HAO, Song MK, Rhee HW (1997) Electrode reaction of Desulfovibrio desulfuricans modified with organic conductive compounds. Biotecnol. Techn. 11: 145-148.

    Google Scholar 

  • Saffarini DA, Nealson KH (1993) Sequence and genetic characterization of etrA, an fnr analog that regulates anaerobic respiration in Shewanella-putrefaciens MR-1. J. Bacteriol. 175: 7938-7944.

    Google Scholar 

  • Uosaki K, Hill HAO (1981) Adsorption behavior of 4,4'-bipyridyl at a gold/water interface and its role in the electron transfer reaction between cytochrome c and gold electrode. J. Electroanal. Chem. 122: 321-327.

    Google Scholar 

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Authors and Affiliations

  1. Water Environment Research Centre, Korea Institute of Science and Technology, 39-1 Hawolgok-dong, c[Sungpook-ku, Seoul , 136-791, Korea

    Byung Hong Kim, Hyung Soo Park, Hyung Joo Kim & Moon Sik Hyun

  2. Division of Applied Life Science, Graduate School of Agriculture, Kyoto University, Sankyo-ku, Kyoto, 606, Japan

    Tokuji Ikeda, Kenji Kano, Kazuyoshi Takagi & Hirosuke Tatsumi

Authors
  1. Byung Hong Kim
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  2. Tokuji Ikeda
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  3. Hyung Soo Park
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  4. Hyung Joo Kim
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  5. Moon Sik Hyun
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  6. Kenji Kano
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  7. Kazuyoshi Takagi
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  8. Hirosuke Tatsumi
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Kim, B.H., Ikeda, T., Park, H.S. et al. Electrochemical activity of an Fe(III)-reducing bacterium, Shewanella putrefaciens IR-1, in the presence of alternative electron acceptors. Biotechnology Techniques 13, 475–478 (1999). https://doi.org/10.1023/A:1008993029309

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  • DOI: https://doi.org/10.1023/A:1008993029309

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