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Potato tubers as a biofactory for recombinant antibodies

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Abstract

Potato tubers have been successfully used for high-level production of a recombinant single-chain Fv (ScFv) antibody. Ubiquitous high-level expression was achieved under control of the CaMV 35S promoter through retention of the scFv protein in the endoplasmic reticulum. Recombinant antibodies accumulated up to 2% of total soluble tuber protein. After 1.5 years of tuber storage at 4 °C still half of the amount of scFv present in freshly harvested tubers was detectable. Its specific activity did not decrease during tuber storage. Recombinant protein could be efficiently purified from crude extracts by affinity chromatography.

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Author notes

  1. Olga Artsaenko

    Present address: Chemical and Biochemical Engineering, UMBC, Baltimore, MD, 21228, USA

  2. Ulrike Fiedler

    Present address: Institut für Biotechnologie, Martin-Luther-Universität Halle-Wittenberg, Fachbereich Biochemie/Biotechnologie, Kurt-Mothes-Str. 3, 06120, Germany

Authors and Affiliations

  1. Institut für Pflanzengenetik und Kulturpflanzenforschung, Corrensstr. 3, 06466, Gastersleben, Germany

    Olga Artsaenko, Ulrike Fiedler & Udo Conrad

  2. Federal Centre for Breeding Research on Cultivated Plants, Institute for Breeding Methods in Vegetables, Neuer Weg 22/23, 06484, Quedlinburg, Germany

    Barbara Kettig & Klaus Düring

Authors
  1. Olga Artsaenko
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  2. Barbara Kettig
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  3. Ulrike Fiedler
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  4. Udo Conrad
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  5. Klaus Düring
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Artsaenko, O., Kettig, B., Fiedler, U. et al. Potato tubers as a biofactory for recombinant antibodies. Molecular Breeding 4, 313–319 (1998). https://doi.org/10.1023/A:1009676832273

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  • DOI: https://doi.org/10.1023/A:1009676832273

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