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Immobilization of invertase on a new type of macroporous glycidyl methacrylate

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Abstract

Invertase was immobilized via its carbohydrate moiety. The immobilized enzyme has a specific activity of 5500 IU g−1, with 45% activity yield on immobilization. In a packed bed reactor, 90% 2.5 M sucrose was converted at a flow rate of 4 bed volumes h−1. The obtained specific productivity at 40 °C of 3 kg l−1 h−1 is the best one so far. Long-term stability was 290 days in 2.5 M sucrose at 40 °C and at a flow rate of 3 bed volumes h−1.

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Authors and Affiliations

  1. Department of Biochemistry, Faculty of Chemistry, Studentski trg 12-16, 11000, Belgrade, Yugoslavia

    Radivoje Prodanović & Zoran Vujčić

  2. Department of Polymer Science, Faculty of Technology and Metallurgy, Karnegijeva 4, 11000, Belgrade, Yugoslavia

    Slobodan Jovanović

Authors
  1. Radivoje Prodanović
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  2. Slobodan Jovanović
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  3. Zoran Vujčić
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Correspondence to Zoran Vujčić.

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Prodanović, R., Jovanović, S. & Vujčić, Z. Immobilization of invertase on a new type of macroporous glycidyl methacrylate. Biotechnology Letters 23, 1171–1174 (2001). https://doi.org/10.1023/A:1010560911400

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  • DOI: https://doi.org/10.1023/A:1010560911400

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