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The Use of a GUS Transformant of Trichoderma Harzianum, Strain T3a, to Study Metabolic Activity in the Spermosphere and Rhizosphere Related to Biocontrol of Pythium Damping-off And Root Rot

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Abstract

The activity of Trichoderma harzianum in the spermosphere and rhizosphere of different plant species was studied by use of a beta-glucuronidase (GUS) transformant (strain T3a). Hereby, direct observation of micro-habitats supporting metabolic activity of T. harzianum is reported. Germination of conidia and mycelial growth were not supported by exudates from healthy roots of various ages. Instead, growth and activity of T. harzianum depended on access to dead organic substrates such as seed coats, decaying roots, and wounds, including those caused by infecting pathogens. A correlation between the GUS activity of T. harzianum and the biomass of Pythium ultimum in infected roots was established. On the basis of our observations, we suggest that the biocontrol ability of T. harzianum involves competition with the pathogen for substrates including the seed coat, and wounded or infected root tissue.

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References

  • Ahmad JS and Baker R (1987a) Rhizosphere competence of Trichoderma harzianum. Phytopathology 77: 182-189

    Google Scholar 

  • Ahmad JS and Baker R (1987b) Competitive saprophytic ability and cellulolytic activity of rhizosphere-competent mutants of Trichoderma harzianum. Phytopathology 77: 358-362

    Google Scholar 

  • Ahmad JS and Baker R (1988) Implication of rhizosphere competence of Trichoderma harzianum. Canadian Journal of Microbiology 34: 229-234

    Google Scholar 

  • Baker R (1978) Inoculum potential. In: Horsfall JG and Cowling EB (eds) Plant Disease. An advanced treatise. Vol. 2. How disease develops in populations (pp 137-157) Academic Press, New York

    Google Scholar 

  • Barton R (1961) Saprophytic activity of Pythium mamillatum in soils. II. Factors restricting P. mamillatum to pioneer colonization of substrates. Transactions of British Mycological Society 44: 105-118

    Google Scholar 

  • Chao WL, Nelson EB, Harman GE and Hoch HC (1986) Colonization of the rhizosphere by biological control agents applied to seeds. Phytopathology 76: 60-65

    Google Scholar 

  • Chen W, Hoitink HAJ, Schmitthenner AF and Tuovinen OH (1988) The role of microbial activity in suppression of damping-off caused by Pythium ultimum. Phytopathology 78: 314-322

    Google Scholar 

  • Chérif M, Benhamou N and Bélanger RR (1991) Ultrastructural and cytochemical studies of fungal development and host reactions in cucumber plants infected by Pythium ultimum. Physiology and Molecular Plant Pathology 39: 353-375

    Google Scholar 

  • Chet I (1987) Trichoderma-application, mode of action, and potential as a biocontrol agent of soil-borne plant pathogenic fungi. In: Chet I (ed) Innovative Approaches to Plant Disease Control (pp137-160) Wiley & Sons, New York

    Google Scholar 

  • Cook RJ and Baker KF (1983) The nature and practice of biological control of plant pathogens. The American Phytopathological Society, St. Paul, Minnesota. 539 pp

    Google Scholar 

  • Di Pietro A, Lorito M, Hayes CK, Broadway RM and Harman GE (1993) Endochitinase from Gliocladium virens: Isolation, characterization, and synergistic antifungal activity in combination with gliotoxin. Phytopathology 83: 308-313

    Google Scholar 

  • Elad Y and Chet I (1987) Possible role of competition for nutrients in biocontrol of Pythium damping-off by bacteria. Phytopathology 77: 190-195

    Google Scholar 

  • Eparvier A and Alabouvette C (1994) Use of ELISE and GUStransformed strains to study competition between pathogenic and non-pathogenic Fusarium oxysporum for root colonization. Biocontrol Science and Technology 4: 35-47

    Google Scholar 

  • Fravel DR and Keinath AP (1991) Biocontrol of soilborne plant pathogens with fungi. In: Keister DL and Cregan PB (eds) The rhizosphere and plant growth (pp 237-243) Kluwer Academic Publishers, The Netherlands

    Google Scholar 

  • Green H and Jensen DF (2000) Disease progression by active mycelial growth and biocontrol of Pythium ultimum var. ultimum studied by using a rhizobox system. Phytopathology 90: 1049-1055

    Google Scholar 

  • GreenHand Jensen DF(1995) Atool for monitoring Trichoderma harzianum: II. The use of a GUS transformant for ecological studies in the rhizosphere. Phytopathology 85: 1436-1440

    Google Scholar 

  • Jensen DF and Wolffhechel H (1995) The use of fungi, particularly Trichoderma spp. and Gliocladium spp., to control root rot and damping-off diseases. In: Hokkanen H and Lynch JM (eds) Biocontrol Agents: Benefits and Risks (pp 177-189) Cambridge University Press, Cambridge

    Google Scholar 

  • Lifshitz R, Windham MT and Baker R (1986) Mechanism of biological control of preemergence damping-off of pea by seed treatment with Trichoderma spp. Phytopathology 76: 720-725

    Google Scholar 

  • Lo C-T, Nelson EB, Hayes CKand Harman GE(1998) Ecological studies of transformed Trichoderma harzianum strain 1295-22 in the rhizosphere and on the phylloplane of creeping bentgrass. Phytopathology 88: 129-136

    Google Scholar 

  • Lockwood JL (1977) Fungistasis in soils. Biological Reviews 52: 1-43

    Google Scholar 

  • Lumsden RD, Carter JP, Whipps JM and Lynch JM (1990) Comparison of biomass and viable propagule measurements in the antagonism of Trichoderma harzianum against Pythium ultimum. Soil Biology and Biochemistry 22: 187-194

    Google Scholar 

  • Nelson EB (1991) Exudate molecules initiating fungal responses to seeds and roots. In: Keister DL and Cregan PB (eds) The Rhizosphere and Plant Growth (pp 197-209) Kluwer Academic Publishers, The Netherlands

    Google Scholar 

  • Papavizas GC (1982) Survival of Trichoderma harzianum in soil and in pea and bean rhizosphere. Phytopathology 71: 121-125

    Google Scholar 

  • Papavizas GC (1985) Trichoderma and Gliocladium: biology, ecology, and potential for biocontrol. Annual Reviews of Phytopathology 23: 23-54

    Google Scholar 

  • Paulitz TC (1991) Effect of Pseudomonas putida on the stimulation of Pythium ultimum by seed volatiles of pea and soybean. Phytopathology 81: 1282-1287

    Google Scholar 

  • Rosendahl CN and Olson LW 1992 An in vivo screening method for antifungal activity against the plant pathogen Pythium ultimum Trow. Journal of Phytopathology 134: 324-328

    Google Scholar 

  • Schirmböck M, Lorito M, Wang Y-L, Hayes CK, Arisan-Atac I, Scala F, Harman GE and Kubicek CP (1994) Parallel formation and synergism of hydrolytic enzymes and peptaibol antibiotics, molecular mechanisms involved in the antagonistic action of Trichoderma harzianum against phytopathogenic fungi. Applied Environmental Microbiology 60: 4364-4370

    Google Scholar 

  • Sivan A and Chet I (1989) The possible role of competition between Trichoderma harzianum and Fusarium oxysporum on rhizosphere colonization. Phytopathology 79: 198-203

    Google Scholar 

  • Sivan A and Harman GE (1991) Improved rhizosphere competence in a protoplast fusion progeny of Trichoderma harzianum. Journal of Genetic Microbiology 137: 23-29

    Google Scholar 

  • Sivan A, Elad Y and Chet I (1984) Biological control effects of a new isolate of Trichoderma harzianum on Pythium aphanidermatum. Phytopathology 74: 498-501

    Google Scholar 

  • Tedla T and Stanghellini ME (1992) Bacterial population dynamics and interactions with Pythium aphanidermatum in intact rhizosphere soil. Phytopathology 82: 652-656

    Google Scholar 

  • Thrane C, Tronsmo A and Jensen DF (1997) {Endo-1,3-β-glucanase and cellulase from Trichoderma harzianum: purifi-cation and partial characterization, induction of and biological activity against plant pathogenic Pythium spp. European Journal of Plant Pathology 103: 331-334

    Google Scholar 

  • Thrane C, Lübeck M, Green H, Degefu Y, Allerup S, Thrane U and Jensen DF (1995) A tool for monitoring Trichoderma harzianum: I. Transformation with the GUS gene by protoplast technology. Phytopathology 85: 1428-1435

    Google Scholar 

  • Weller DM (1983) Colonization of wheat roots by a fluorescent pseudomonad suppressive to take-all. Phytopathology 73: 1548-1553

    Google Scholar 

  • Widden SE and Scattolin V (1988) Competitive interactions and ecological strategies of Trichoderma species colonizing spruce litter. Mycologia 80: 795-803

    Google Scholar 

  • Wilhelm S and Nelson PE (1970) A concept of rootlet health of strawberries in pathogen-free field soil achieved by fumigation. In: Toussoun TA, Bega RV and Nelson PE (eds) Root Diseases and Soil-borne Pathogens (pp 208-215) University of California Press, Berkeley

    Google Scholar 

  • Wolffhechel H (1989) Fungal antagonists of Pythium ultimum isolated from a disease-suppressive Sphagnum peat. Växtskyddsnotiser 53: 7-11

    Google Scholar 

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  1. Nina Heiberg

    Present address: Department of Plant Biology, Plant Pathology Section, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Copenhagen, Denmark

Authors and Affiliations

  1. Department of Plant Biology, Plant Pathology Section, The Royal Veterinary and Agricultural University, Thorvaldsensvej 40, DK-1871, Frederiksberg C, Copenhagen, Denmark

    Helge Green, Kirsten Lejbølle & Dan Funck Jensen

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  1. Helge Green
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  2. Nina Heiberg
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  3. Kirsten Lejbølle
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  4. Dan Funck Jensen
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Green, H., Heiberg, N., Lejbølle, K. et al. The Use of a GUS Transformant of Trichoderma Harzianum, Strain T3a, to Study Metabolic Activity in the Spermosphere and Rhizosphere Related to Biocontrol of Pythium Damping-off And Root Rot. European Journal of Plant Pathology 107, 349–359 (2001). https://doi.org/10.1023/A:1011274432090

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