Abstract
A cell suspension culture of Tabernaemontana divaricata, that had lost alkaloid production, was still capable of producing a similar pattern of alkaloids as directly after its initiation. When fed with early precursors, such as tryptamine and loganin, 57% of the precursors was converted into indole alkaloids such as strictosidine, vallesamine, O-acetylvallesamine and voaphylline. Apparently most of the cell factory has remained stable during the many years of subculturing. Only an early step of the biosynthesis the flux seems to be diverted to other pathways.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Dagnino D,Schripsema J,Peltenburg A,Verpoorte R,Teunis K (1991) Investigation of the indole alkaloids present in Tabernaemontana divaricata cell suspension culture by capillary gas chromatography. J. Nat. Prod. 54: 1558-1563.
Dagnino D,Schripsema J,Verpoorte R (1993a) Alkaloid metabolism in Tabernaemontana divaricata cell suspension cultures. Phytochemistry 32: 325-329.
Dagnino D,Schripsema J,Verpoorte R (1993b) Comparison of terpenoid indole alkaloid production and degradation in two cell lines of Tabernaemontana divaricata. Plant Cell Rep. 13: 95-98.
Dagnino D,Schripsema J,Verpoorte R (1994) Terpenoid indole alkaloid biotransformation of suspension cultures of Tabernaemontana divaricata. Phytochemistry 35: 671-676.
Dagnino D,Schripsema J,Verpoorte R (1995) Terpenoid indole alkaloid biosynthesis and enzyme activities in two cell lines of Tabernaemontana divaricata (Apocynaceae). Phytochemistry 39: 341-349.
Deus-Neumann B,Zenk MH (1984) Instability of indole alkaloids production in Catharanthus roseus cells suspension culture. Planta Med. 50: 427-431.
Murashige T,Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473-479.
Ohta S,Verpoorte R (1992) Some accounts of variation (heterogeneity and/or instability) in secondary metabolites production by plant cell cultures. Annu. Rep. Nat. Science Home Econ. 32: 9-23.
Pawelka KH,Stöckigt J (1983) Indole alkaloids from cell suspension culture of Tabernaemontana divaricata and Tabernaemontana iboga. Plant Cell Rep. 2: 105-107.
Schripsema J,Verpoorte R (1992) Search for factors related to the indole alkaloid production in cell suspension cultures of Tabernaemontana divaricata. Planta Med. 58: 245-250.
Schripsema J,Dagnino D,dos Santos R,Verpoorte R (1994) Breakdown of indole alkaloids in suspension cultures of Tabernaemontana divaricata and Catharanthus roseus. Plant Cell Tiss. Org. Cult. 38: 301-307.
Sierra MI,Van der Heiden R,Van der Leer T,Verpoorte R (1991) Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture. Ph.D. Thesis. Leyden University, The Netherlands.
Sierra M,van der Heijden R,van der Leer T,Verpoorte R (1992) Stability of alkaloid production in cell suspension cultures of Tabernaemontana divaricata during long-term subculture. Plant Cell Tiss. Org. Cult. 28: 59-68.
Taesotikul T (1997) Tabernaemontana pandacaqui Poir., a traditional Thai medicinal plant. Ph.D. Thesis, Leyden University, The Netherlands.
Taesotikul T,Panthong A,Kanjanapothi D,Verpoorte R,Scheffer JJC (1989) Hippocratic screening of ethanolic extracts from two Tabernaemontana species. J. Ethnopharmacol. 27: 99-106.
van der Heijden R,Brouwer RL,Verpoorte R, Wijnsma, Van Beek TA,Harkes PAA,Svendsen AB (1986) Indole alkaloids from a callus culture of Tabernaemontana elegans. Phytochemistry 25: 393-397.
van der Heijden R,Hermans-Lokkerbol A,de Kool LPJ,Lamping PJ,Harkes PAA,Verpoorte R (1988) Accumulation of indole al1 kaloids in a suspension culture of Tabernaemontana divaricata. Planta Med. 54: 393-397.
van der Heijden R,van der Graaf GM,Pennings EJM,Verpoorte R (1990) Formation and degradation of some aspidospermatan indole alkaloids in suspension cultures of Tabernaemontana divaricata. Plant Physiol. Biochem. 28: 351-358.
Whitmer S,Canel C,Hallard D,Goncalves C,Verpoorte R (1998) Influence of precursor availability on alkaloid accumulation by transgenic cell line of Catharanthus roseus. Plant Physiol. 116: 853-857.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Lucumi, E., Luczkiewicz, M., Vera, A. et al. Alkaloid formation in cell suspension cultures of Tabernaemontana divaricata after feeding of tryptamine and loganin. Biotechnology Letters 23, 1691–1696 (2001). https://doi.org/10.1023/A:1012452313625
Issue Date:
DOI: https://doi.org/10.1023/A:1012452313625