Abstract
A collection of thermophilic strains of the genus Bacillus was made. The strains were screened for antimicrobial activity. Strains VK2 and VK21 isolated from thermal springs of the Kamchatka Peninsula, and antagonistic to several gram-positive bacterial species were chosen for further investigation of antibiotics produced by them. Restriction analysis of DNA coding for 16S rRNA showed that both strains can be assigned to Bacillus licheniformis. It was shown that the lytic activity of strains VK2 and VK21 was not related to the synthesis of hydrolytic enzymes. The maximum level of antimicrobial activity in the growth medium was found to correspond to the beginning of the stationary growth phase. Addition of manganese sulfate induced sporulation and altered significantly the time course of antibiotic production in both strains. Active metabolites were extracted with n-butanol. They survived boiling for 30 min and were resistant to trypsin and chymotrypsin but were partly hydrolyzed by pronase. They were stable at a pH range of 2.0–9.0.
Similar content being viewed by others
REFERENCES
Shemyakin, M.M., Khokhlov, A.S., Kolosov, M.N., et al., Khimiya antibiotikov (Chemistry of Antibiotics), Moscow: USSR Academy of Sciences, 1961, vols. 1, 2.
Kleinkauf, H. and von Dohren, H., Eur. J. Biochem., 1990, vol. 192, no. 1, pp. 1-15.
Zuber, P.A. and Marahiel, M. A., Biotechnology of Antibiotics, Strohl, W., Ed., New York: Marcel Dekker, 1997, pp. 187-216.
Egorov, N.S., Osnovy ucheniya ob antibiotikakh (Basic Principles of the Theory of Antibiotics), Moscow: Vysshaya Shkola, 1986.
Egorov, N.S., Silaev, A.B., Katrukha, G.S., et al., Antibiotiki-polipeptidy (Polypeptide Anyibiotics), Moscow: Mosk. Gos. Univ., 1987.
Aguilar, A., FEMS Microbiol. Rev., 1996, vol. 18, nos. 2-3, pp. 89-92.
Ghauri, M. and Johnson, D., FEMS Microbiol. Ecol., 1991, vol. 85, pp. 327-331.
Aagaard, C., Leviev, I., Aravalli, R.N., et al., FEMS Microbiol. Rev., 1996, vol. 18, nos. 2-3, pp. 93-104.
Kengen, S.W.M., Stams, A.J.M., and De Vos, W.M., FEMS Microbiol. Rev., 1996, vol. 18, nos. 2-3, pp. 119-138.
Kosmachev, A.E., Khokhlova, Yu.M., and Kalmykova, G.Ya., Mikrobiologiya, 1965, vol. 34, pp. 437-441.
Saito, M., Matsuura, I., and Okazaki, H., J. Antibiotics, 1979, vol. 32, no. 11, pp. 1210-1212.
Egorov, N.S., Vestn. Mos. Gos. Univ., Ser. Biol., 1999, no. 4, pp. 38-49.
Pichard, B., Larue, J.-P., and Thouvenot, D., FEMS Microbiol. Lett., 1995, vol. 133, no. 3, pp. 215-218.
Sambrook, J., Fritsch, E.F., and Maniatis, T., Molecular Cloning: A Laboratory Manual, New York: Cold Spring Harbor Lab., 1989.
Kvasnikov, E.I. and Tin'yanova, N.Z., Mikrobiol. Zh., 1981, vol. 43, no. 2, pp. 152-155.
Woese, C.R., Stakebrandt, E., Nacke, T.G., and Fox, G.E., Syst. Appl. Bacteriol., 1985, vol. 60, pp. 143-151.
Pavlova, I.N., Tin'yanova, N.Z., and Zholner, L.G., Mikrobiol. Zh. (Kiev), 1991, vol. 53, no. 1, pp. 84-89.
Kurusu, K. and Ohba, K., J. Antibiotics, 1987, vol. 40, no. 11, pp. 1506-1514.
Nefelova, M.V., Ermakova, G.N., Gulevskaya, S.A., and Egorov, N.S., Mikrobiologiya, 1981, vol. 50, no. 4, pp. 677-682.
Lee, S.G., Littau, V., and Lipmann, F., J. Cell Biol., 1975, vol. 66, no. 2, pp. 233-242.
Schaeffer, P., Bacteriol. Rev., 1969, vol. 33, no. 1, pp. 48-71.
Korshunov, V.V. and Egorov, N.S., Mikrobiologiya, 1962, vol. 3, pp. 515-519.
Kashkin, P.N., Bezborodov, A.M., Elinov, N.P., and Tsyganov, V.A., Antibiotiki (Antibiotics), Leningrad: Meditsina, 1970.
Egorov, N.S., Vypiyach, A.N., Zharikova, G.G., et al., Antibiotiki i ikh produtsenty (Antibiotics and Their Producers), Moscow: Nauka, 1975, pp. 187-196.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Esikova, T.Z., Temirov, Y.V., Sokolov, S.L. et al. Secondary Antimicrobial Metabolites Produced by Thermophilic Bacillus spp. Strains VK2 and VK21. Applied Biochemistry and Microbiology 38, 226–231 (2002). https://doi.org/10.1023/A:1015463122840
Issue Date:
DOI: https://doi.org/10.1023/A:1015463122840