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Vitrification of Human Blastocysts Using the Cryoloop Method: Successful Clinical Application and Birth of Offspring

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Abstract

Purpose : The purpose was to investigate vitrification of human blastocysts using the cryoloop.

Methods : Thirteen couples elected to have blastocysts cryopreserved via vitrification (N = 54 blastocysts). Embryos were exposed to two vitrification solutions, pipetted onto the cryoloop then plunged into liquid nitrogen. On the day of embryo replacement, blastocysts were warmed by passage through three dilution media, and rinsed into culture medium.

Results : Four couples returned for cryopreserved embryo replacement. Fifteen blastocysts were warmed; all 15 (15/15; 100%) were recovered. Fifteen (15/15; 100%) blastocysts were deemed viable, and 13 were replaced. Two replacements did not result in pregnancy; one resulted in clinical pregnancy with a blighted ovum; one resulted in clinical pregnancy with the live, term birth of a healthy male infant. Two of 13 embryos implanted yielding an implantation rate of 15.4%.

Conclusions : These preliminary data suggest that blastocysts can be successfully vitrified using the cryoloop method.

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REFERENCES

  1. Vajta G: Vitrification of the oocytes and embryos of domestic animals. Animal Reprod Sci 2000;60/61:357-364

    Google Scholar 

  2. Lane M, Bavister BD, Lyons EA, Forest KT: Containerless vitrification of mammalian oocytes and embryos. Nat Biotechnol 1999;17:1234-1236

    Google Scholar 

  3. Dobrinsky JR, Pursel VG, Long CR, Johnson LA: Birth of piglets after transfer of embryos cryopreserved by cytoskeletal stabilization and vitrification. Biol Reprod 2000;62:564-570

    Google Scholar 

  4. Lane M, Schoolcraft WB, Gardner DK: Vitrification of mouse and human blastocysts using a novel cryoloop container-less technique. Fertil Steril 1999;72:1073-1078

    Google Scholar 

  5. Choi DH, Chung HM, Lim JM, Ko JJ, Yoon TK, Cha KY: Pregnancy and delivery of healthy infants developed from vitrified blastocysts in an IVF-ET program. Fertil Steril 2000;74:838-839

    Google Scholar 

  6. Yokota Y, Sato S, Yokota M, Ishikawa Y, Makita M, Asada T, Araki Y: Successful pregnancy following blastocyst vitrification. Hum Reprod 2000;15:1802-1803

    Google Scholar 

  7. Yokota Y, Sato S, Yokota M, Yokota H, Araki Y: Birth of a healthy baby following vitrification of human blastocysts. Fertil Steril 2001;75:1027-1029

    Google Scholar 

  8. Mukaida T, Nakamura S, Tomiyama T, Wada S, Kasai M, Takahashi K: Successful birth after transfer of vitrified human blastocysts with use of a cryoloop containerless technique. Fertil Steril 2001;76:618-620

    Google Scholar 

  9. Han TS, Tucker M, Graham J, Carter J, Mottla G, Stillman RJ: Towards convenient blastocyst vitrification. Fertil Steril 2001; 76(Suppl 1):S119

    Google Scholar 

  10. Yeoman RR, Gerami-Naini B, Mitalipov S, Nusser KD, Widmann-Browning AA, Wolf DP: Cryoloop vitrification yields superior survival of Rhesus monkey blastocysts. Hum Reprod 2001;16:1965-1969

    Google Scholar 

  11. Bard PE, Barad DH, Feichtinger W: Ultrarapid freezing (URF) of mouse and human preembryos: A modified approach. J In Vitro Fert Embryo Transfer 1990;2:59-64

    Google Scholar 

  12. Gordts S, Roziers P, Campo R, Noto V: Survival and pregnancy outcome after ultrarapid freezing of human embryos. Fertil Steril 1990;53:469-472

    Google Scholar 

  13. Lai AC, Lin BP, Chang CC, Tsai HD, Hwang VW, Lo HY: Pregnancies after transfer of ultrarapidly frozen human embryos. J Assist Reprod Genet 1996;13:625-628

    Google Scholar 

  14. Hsieh YY, Tsai HD, Chang CC, Chang CC, Lo HY, Lai AC: Ultrarapid cryopreservation of human embryos: Experience with 1,582 embryos. Fertil Steril 1999;72:253-256

    Google Scholar 

  15. Van DerElst J, Camus M, Van de Abbeel E, Maes R, Devroey P, Van Steirtegem AC: Prospective randomized study on the cryopreservation of human embryos with dimethylsulfoxide or 1,2-propanediol protocols. Fertil Steril 1995;63:92-100

    Google Scholar 

  16. Noto V, Campo R, Roziers P, Gordts S: Fluorescein diacetate assessment of embryo viability after ultrarapid freezing of human multipronucleate embryos. Fertil Steril 1991;55:1171-1175

    Google Scholar 

  17. Noto V, Campo R, Roziers P, Swinnen K, Vercruyssen M, Gordts S: Mitochondrial distribution after fast embryo freezing. Hum Reprod 1993;8:2115-2118

    Google Scholar 

  18. Park SP, Kim EY, Oh JH, Nam JK, Lee KS, Park SY, Park EM, Yoon SH, Chung KS, Lim JH: Ultra-rapid freezing of human multipronuclear zygotes using electron microscopy grids. Hum Reprod 2000;15:1787-1790

    Google Scholar 

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Correspondence to Michael L. Reed.

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Reed, M.L., Lane, M., Gardner, D.K. et al. Vitrification of Human Blastocysts Using the Cryoloop Method: Successful Clinical Application and Birth of Offspring. J Assist Reprod Genet 19, 304–306 (2002). https://doi.org/10.1023/A:1015789532736

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  • DOI: https://doi.org/10.1023/A:1015789532736

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