Abstract
Efficient and rapid tissue culture systems were developed for Piper longum, an important medicinal plant, through shoot tip multiplication and direct regeneration. Multiple shoots were induced from shoot tips cultured on agar-based Murashige and Skoog (MS) medium containing 4.44–22.19 μM benzyladenine (BA) and 4.64–13.9 μM kinetin (K). Maximum number of shoots were induced with 8.9 μM BA and 4.64 μM K. Adventitious shoot regeneration from leaf segments was achieved on MS containing 3.6–22.19 μM BA along with 3.31–12.4 μM picloram (P). Shoot differentiation occurred directly from the leaf bases without intermediale callus formation. Maximum shoot buds were obtained on MS medium with 17.76 μM BA and 8.28 μM P. Elongated shoots were separated and rooted in MS supplemented with 2.46 μM indole butyric acid (IBA). Plantlets, thus developed were established in soil.
This is a preview of subscription content, log in via an institution to check access.
Similar content being viewed by others
References
Bhat SR Chandel KPS & Malik SK (1995) Plant regeneration from various explants of cultivated Piper species. Plant Cell Rep. 14: 398–402
Carneiro LA, Araujo RFG, Brito GJM, Fonseca MHPB, Coasta A, Crocomo OJ & Mansur E (1999) In vitro regeneration from leaf explants of Neoregelia cruenta (R. Graham) L.B. Smith, an endemic Bromeliad from Eastern Brazil. Plant Cell Tiss. Org. Cult. 55: 79–83
Hosoki T & Asahira T (1980) In vitro propagation of Bromeliads in liquid culture. Hort Science 15: 603–604
Kukulczanka K & Czastka B (1989) Propagation of some species of the Bromeliaceae family cultured in vitro. Acta Hort. 251: 167-181
Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue culture. Physiol. Plant. 15: 473–497
Nair KKN (2000) Manual of Nonwood Forest Produce Plants of Kerala (pp 268–270). Kerala Forest Research Institute, Kerala, India
Philip S, Banerjee NS & Das MR (2000) Genetic variation and micropropagation in three varieties of Piper longum L. Current Science 78: 169–173
Sarasan V & Nair GM (1991) Tissue culture of medicinal plants: morphogenesis, direct regeneration and somatic embryogenesis. In: Prakash J & Pierik RLM (eds) Horticulture - New Technologies & Applications (pp 237–240). Kluwer Academic Publishers, Dordrecht, The Netherlands
Sarasan V, Thomas E, Lawrence B & Nair GM (1993) Plant regeneration in Piper longum L. ( Piperaceae) through direct and indirect shoot development. J. spices arom. Crops 2: 34–40
Sivarajan VV & Balachandran I (1994) Ayurvedic Drugs and their Plant Sources (pp 374–376). Oxford and IBH Pubishing Co. Pvt. Ltd.
Author information
Authors and Affiliations
Corresponding author
Rights and permissions
About this article
Cite this article
Soniya, E., Das, M. In vitro micropropagation of Piper longum – an important medicinal plant. Plant Cell, Tissue and Organ Culture 70, 325–327 (2002). https://doi.org/10.1023/A:1016561521050
Issue Date:
DOI: https://doi.org/10.1023/A:1016561521050