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Coating efficiency of a 1 -acid glycoprotein in competitive enzyme immunosorbent assays with antigen immobilized on the solid phase

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Biotechnology Techniques

Abstract

Coating efficiency of rat and human serum a 1 -acid glycoprotein (a 1 -AGP) was investigated for competitive enzyme immunosorbent assays with antigen immobilized on the solid phase by using different pHs and buffers. Blocking materials and pH of coating buffer had a marked influence on the amount of a 1 -AGP that binds to plate. Usually, carbonate buffer is used at pH 9.6 or 9.0, but phosphate buffered saline (PBS) at pH 7.2 can be used for an effective coating. At pH 7.2, coating of a 1 -AGP in Tris buffered saline was five - tenfold as effective as in PBS and phosphate buffer. Blocking of uncoated surface with casein was ten - twenty times as effective as with fetal bovine serum albumin for coating of a 1 -AGP.

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Authors and Affiliations

  1. Department of Radiation Biology, Korea Atomic Energy Research Institute, P.O. Box 105, Yusong Taejon, 305-600, Korea

    In Gyu Kim & Seon Young Park

Authors
  1. In Gyu Kim
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  2. Seon Young Park
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Kim, I.G., Young Park, S. Coating efficiency of a 1 -acid glycoprotein in competitive enzyme immunosorbent assays with antigen immobilized on the solid phase. Biotechnology Techniques 11, 335–338 (1997). https://doi.org/10.1023/A:1018479630885

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  • DOI: https://doi.org/10.1023/A:1018479630885

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