Abstract
The possible role of surface energy thermodynamics in mucoadhesion was investigated with Polycarbophil and pig intestinal mucosa. In separate experiments, the surface energy parameters of the substrate (mucosa) and the adhesive (polymer film) were determined by contact angle measurements on captive air/octane bubbles in three physiologically relevant test fluids (isotonic saline, artificial gastric fluid, and artificial intestinal fluid). Whereas the swollen Polycarbophil films were relatively hydrophilic as indicated by small water contact angles (22, 23, and 16°), the water contact angles measured on mucosal tissue were significantly larger (61, 48, and 57°). Hence, mucus was found to possess an appreciable hydrophobicity. The measured adhesive performance (force of detachment) between Polycarbophil and pig small intestinal mucosa was highest in non-buffered saline medium, intermediate in gastric fluid, and minimal in intestinal fluid. In agreement with this trend, the mismatch in surface polarities between substrate and adhesive, calculated from the contact angle data, increased in the same order.
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REFERENCES
R. Gurny and H. E. Junginger. Bioadhesion—Possibilities and Future Trends, Wiss. Verlagsgesellschaft, Stuttgart, 1990.
N. A. Peppas and P. A. Buri. Surface, interfacial and molecular aspects of polymer bioadhesion on soft tissues. J. Control. Release 2:257–275 (1985).
G. Ponchel, F. Touchard, D. Duchêne, and N. A. Peppas. Bioadhesive analysis of controlled-release systems. I. Fracture and interpenetration analysis in poly(acrylic acid) containing systems. J. Control Release 5:129–141 (1987).
B. O. Bateup. Surface chemistry and adhesion. Int. J. Adhes. Adhes. 233–239 (1981).
H. W. Kammer. Adhesion between polymers. Acta Polymer. 34:112–118 (1983).
D. Eagland. Adhesion and adhesive performance: The scientific background. Endeavour 12:179–184 (1988).
R. E. Baier, E. G. Shafrin, and W. A. Zisman. Adhesion: Mechanisms that assist or impede it. Science 162:1360–1368 (1968).
H. J. Busschler, A. H. Weerkamp, H. C. van de Mei, A. W. J. van Pelt, H. P. de Jong, and J. Arends. Measurement of the surface free energy of bacterial cell surfaces and its relevance for adhesion. Appl. Env. Microbiol. 48:980–983 (1984).
C.-M. Lehr, J. A. Bouwstra, J. J. Tukker, and H. E. Junginger. Intestinal transit of bioadhesive microspheres in an in situ loop in the rat—a comparative study with copolymers and blends based on poly(acrylic acid). J. Control. Release 13:51–62 (1990).
F. J. Holly and M. J. Refojo. Wettability of Hydrogels I. Poly(2-hydroxyethyl methacrylate). J. Biomed. Mater. Res. 9:315–326 (1975).
J. D. Andrade, R. N. King, D. E. Greggonis, and D. L. Coleman. Surface characterization of poly(hydroxyethyl methacrylate) and related polymers. I. Contact angle methods in water. J. Polym. Sci. Polym. Symp. 66:313–336 (1979).
W. C. Hamilton. A technique for the characterization of hydrophilic solid surfaces. J. Colloid Interf. Sci. 40:219–222 (1972).
F. M. Fowkes. Attractive forces at interfaces. Ind. Eng. Chem. 56(12):40–52 (1964).
D. K. Owens and R. C. Wendt. Estimation of the surface free energy of polymers. J. Appl. Polym. Sci. 13:1741–1747 (1969).
R. C. Weast. Handbook of Chemistry and Physics, 56th ed., CRC Press, Cleveland, OH, 1974.
S. H. Yuk and M. S. Jhon. Contact angles on deformable solids. J. Colloid Interf. Sci. 110:252–257 (1986).
A. G. Mikos and N. A. Peppas. Measurement of the surface tension of mucin solutions. Int. J. Pharm. 53:1–5 (1989).
A. Baszkin, J. E. Proust, P. Monsenego, and M. M. Boissonade. Wettability of polymers by mucin aqueous solutions. Biorheology 27:503–514 (1990).
A. W. Neumann and R. J. Good. Techniques of measuring contact angles. In R. J. Good and R. R. Stromberg (eds.), Surface and Colloid Science, Vol. 11, Plenum Press, New York, 1979, pp. 31–92.
B. A. Hills, B. D. Butler, and L. M. Lichtenberger. Gastric mucosal barrier: Hydrophobic lining to the lumen of the stomach. Am. J. Physiol. 244:G561–G568 (1983).
R. T. Spychal, J. M. Marrero, S. H. Savermuttu, and T. C. Northfield. Measurement of the surface hydrophobicity of human gastrointestinal mucosa. Gastroenterology 97:104–111 (1989).
H. Park and J. R. Robinson. Physico-chemical properties of water insoluble polymers important to mucin/epithelial adhesion. J. Control. Release 2:47–57 (1985).
S. S. Leung and J. R. Robinson. The contribution of anionic polymer structure features to mucoadhesion. J. Control. Release 5:223–231 (1988).
S. S. Leung and J. R. Robinson. Polymer structures features contributing to mucoadhesion. II. J. Control. Release 12:187–194 (1990).
J. D. Smart, I. W. Kellaway, and H. E. C. Wortington. An in-vitro investigation of mucosa adhesive materials for use in controlled drug delivery. J. Pharm. Pharmacol. 36:295–299 (1984).
P. Forget, P. Gazzeri, F. Moreau, M. Sabatier, C. Durandeau, J. P. Merlet, and P. Aumonier. Comprimés mucoadhésifs. Mesure de l'adhésivité in vitro. S.T.P. Pharma 4:176–181 (1988).
S. H. Wu. Polymer Interface and Adhesion, Marcel Dekker, New York, 1982.
H. E. Boddé. Principles of bioadhesion. In R. Gurny and H. E. Junginger (eds.), Bioadhesion—Possibilities and Future Trends, Wiss. Verlagsgesellschaft, Stuttgart, 1990, pp. 44–64.
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Lehr, CM., Bouwstra, J.A., Boddé, H.E. et al. A Surface Energy Analysis of Mucoadhesion: Contact Angle Measurements on Polycarbophil and Pig Intestinal Mucosa in Physiologically Relevant Fluids. Pharm Res 9, 70–75 (1992). https://doi.org/10.1023/A:1018931811189
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DOI: https://doi.org/10.1023/A:1018931811189