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Cross-linked enzyme aggregates with enhanced activity: application to lipases

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Abstract

Seven commercially available microbial lipases were immobilised as their cross-linked enzyme aggregates (CLEAs). Preparations with enhanced activity were obtained by a judicious choice of the precipitant [(NH4)2SO4, 1,2-dimethoxyethane or acetone] and by adding either a crown ether or surfactant, depending on the source of the enzyme. Thus, precipitation of the lipases from Thermomyces lanuginosus and Rhizomucor miehei with (NH4)2SO4 in the presence of SDS, followed by cross-linking with glutaraldehyde, afforded CLEAs with three and two times, respectively, the hydrolytic activity of the native enzymes. Preparations with up to ten times enhanced activity in organic medium were similarly prepared.

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Authors and Affiliations

  1. Laboratory of Biocatalysis and Organic Chemistry, Julianalaan 136, 2628 BL, Delft, The Netherlands

    P. López-Serrano, L. Cao, F. van Rantwijk & R.A. Sheldon

Authors
  1. P. López-Serrano
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  2. L. Cao
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  3. F. van Rantwijk
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  4. R.A. Sheldon
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López-Serrano, P., Cao, L., van Rantwijk, F. et al. Cross-linked enzyme aggregates with enhanced activity: application to lipases. Biotechnology Letters 24, 1379–1383 (2002). https://doi.org/10.1023/A:1019863314646

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  • DOI: https://doi.org/10.1023/A:1019863314646

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