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Association of GPI-Anchored Protein TAG-1 with Src-Family Kinase Lyn in Lipid Rafts of Cerebellar Granule Cells

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Abstract

We have demonstrated that antibody-mediated crosslinking of GPI-anchored TAG-1 induced activation of src-family kinase Lyn and rapid tyrosine phosphorylation of an 80-kDa protein (p80), a putative substrate for Lyn, in the lipid raft fraction prepared from primary cerebellar cultures, suggesting the functional association of TAG-1 with Lyn in lipid rafts of the rat cerebellum. In this study, the association was confirmed using a cDNA expression system. TAG-1-expressing CHO transfectants exhibited enhanced self-aggregation and promoted neurite outgrowth of primary cerebellar cultures as a culture substrate. The anti-TAG-1 antibody co-immunoprecipitated Lyn with TAG-1 and induced co-patching of TAG-1 with Lyn in both TAG-1 and Lyn-expressing CHO transfectants. Density gradient analysis revealed that TAG-1 is present in the lipid raft fraction of the CHO transfectants. Furthermore, pretreatment with a sphingolipid biosynthesis inhibitor ISP-1 reduced the extent of tyrosine phosphorylation of p80 by the antibody-mediated crosslinking of TAG-1. Immunocytochemical study showed that both TAG-1 and Lyn are present in cerebellar granule cells. These observations suggest that TAG-1 associates with Lyn in lipid rafts of rat cerebellar granule cells.

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Authors and Affiliations

  1. Department of Biochemical Cell Research, The Tokyo Metropolitan Institute of Medical Science, Tokyo Metropolitan Organization for Medical Research, Honkomagome, Bunkyo-ku, Tokyo, 113-8613, Japan

    Kohji Kasahara & Yutaka Sanai

  2. “Time's Arrow Biosignaling”, PRESTO, Japan Science and Technology Corporation (JST), Japan

    Kohji Kasahara

  3. Department of BioEngineering, Nagaoka University of Technology, Niigata

    Kazutada Watanabe

  4. Laboratory of Membrane Biochemistry and Biophysics, Graduate School of Biostudies, Kyoto University, Kyoto

    Yasunori Kozutsumi

  5. Supra-Biomolecular System Research, RIKEN Frontier Research System, Naitama

    Yasunori Kozutsumi

  6. Department of Perinatology and Neuroglycoscience, Institute for Developmental Research, Aichi

    Atsuhiko Oohira

  7. Department of Oncology, Institute of Medical Science, University of Tokyo, Tokyo, Japan

    Tadashi Yamamoto

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  1. Kohji Kasahara
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  2. Kazutada Watanabe
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  5. Tadashi Yamamoto
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  6. Yutaka Sanai
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Kasahara, K., Watanabe, K., Kozutsumi, Y. et al. Association of GPI-Anchored Protein TAG-1 with Src-Family Kinase Lyn in Lipid Rafts of Cerebellar Granule Cells. Neurochem Res 27, 823–829 (2002). https://doi.org/10.1023/A:1020265225916

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