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Effect of osmo- and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress

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Abstract

Seven-day-old seedlings obtained from seeds primed with mannitol (4%)and water showed three to four fold more growth with respect to root and shootlength in comparison with seedlings obtained from non-primed seeds. Seedlingswere grown under water deficit stress conditions created by 15% polyethyleneglycol (PEG) 6000 in the medium. Priming of chickpea seeds with NaCl and PEGwasnot effective in increasing seedling growth under these water deficit stressconditions. The activities of amylase, invertases (acid and alkaline), sucrosesynthase (SS) and sucrose phosphate synthase (SPS) were higher in shoots ofprimed seedlings. An increase in the activities of SS, and both the acid andalkaline invertases was also observed in roots of primed seedlings. The twofoldincrease in specific activity of sucrose phosphate synthase was observed incotyledons of primed seedlings. The higher amylase activity in shoots of primedseedlings enhanced the rapid hydrolysis of transitory starch of the shootleading to more availability of glucose for shoot growth and this was confirmedby the low level of starch in shoots of primed seedlings.

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References

  • Amzallag G.N., Lerner H.R. and Poljakoff-Mayber A. 1990. Exogenous ABA as a modulator of the response of sorghum to high Salinity. J. Exp. Bot 54: 1529–1534.

    Google Scholar 

  • Balibrea M.E., Parra M., Bolarin M.C. and Perez-Alfocea F. 1999. Cytoplasmic sucrolytic activity controls tomato fruit growth under salinity. Aust. J. Plant Physiol. 26: 561–568.

    Google Scholar 

  • Boucaud J. and Unger I.A. 1976. Hormonal control of germination under saline conditions of three halophyte taxa in genus Suaeda. Physiol. Plant. 36: 197–200.

    Google Scholar 

  • Cayuela E., Perez-Alfocea A., Caro M. and Bolarin M.C. 1996. Priming of seeds with NaCl induces physiological changes in tomato plants grown under salt stress. Physiol. Plant. 96: 231–236.

    Google Scholar 

  • Davis B.D. 1984. Regulation of α-amylase activity in bean stem tissues. Plant Physiol. 74: 841–845.

    Google Scholar 

  • Dubois M., Gilles K.A., Hamilton J.K., Rebers P.A. and Smith F. 1956. Colorimetric method for determination of sugars and related substances. Anal. Chem. 28: 350–356.

    Google Scholar 

  • Gonzalez A., Gorden A.J., James C.L. and Arrese-Igor C. 1995. The role of sucrose synthase in response of soybean nodules to drought. J. Expt. Bot. 46: 1515–1523.

    Google Scholar 

  • Gupta A.K., Singh J., Kaur N. and Singh R. 1993a. Effect of polyelthylene glycol induced water stress on germination and reserve carbohydrate metabolism in chickpea cultivars differing in tolerance to water deficit. Plant Physiol. Biochem. 31: 369–378.

    Google Scholar 

  • Gupta A.K., Singh J., Kaur N. and Singh R. 1993b. Effect of polyethylene glycol induced water stress on uptake and transport of sugars in chickpea seedlings. Plant Physiol. Biochem. 31: 743–747.

    Google Scholar 

  • Harris D., Joshi A., Khan P.A., Gothkar P. and Sodhi P.S. 1999. On farm seed priming in semi-arid culture: development and evaluation in maize, rice and chickpea in India using participatory methods. Exp. Agric. 35: 15–29.

    Google Scholar 

  • Kaur S., Gupta A.K. and Kaur N. 1998. Gibberellic acid and kinetin partially reverse the effect of water stress on germination and seedling growth. Plant Growth Regul. 25: 29–33.

    Google Scholar 

  • Kaur S., Gupta A.K. and Kaur N. 2000. Effect of GA3, kinetin and indole acetic acid on carbohydrate metabolism in chickpea seedlings germinating under water stress. Plant Growth Regul. 30: 61–70.

    Google Scholar 

  • Kerr P.S., Torres W.K. and Huber S.C. 1987. Resolution of two molecular forms of sucrose phosphate synthase from maize, soybean and spinach leaves. Planta. 170: 515–519.

    Google Scholar 

  • Krishnan H.B., Blanchette J.T. and Okita T.W. 1985. Wheat invertases: Characterization of cell wall and bound forms. Plant Physiol. 78: 241–245.

    Google Scholar 

  • Lee-suskoon K.M., Hyeum J., Beom H.S., Minkyeong K. and Euiho P. 1998. Optimum water potential, temperature and duration for priming of rice seeds. Korean J. Crop. Sci. 43: 1–5.

    Google Scholar 

  • Lowry O.H., Rosebrough N.J., Farr A.L. and Randall R.J. 1951. Protein measurement with folin phenol reagent. J. Biol Chem. 193: 265–275.

    Google Scholar 

  • Miyagi M., Oku H. and Chinen I. 1990. Purification and action pattern on soluble starch of α-amylase from sugarcane leaves. Agric. Biol. Chem. 54: 849–885.

    Google Scholar 

  • Morgan P.W. 1990. Effects of abiotic stresses on plant hormone systems. In: Alscher R.G. and Cumming J.R. (eds), Stress Response in Plants: Adaptation and Acclimation Mechanism. Wiley-Liss, New York.

    Google Scholar 

  • Murashige T. and Skoog F. 1962. A revised medium for rapid growth and bioassay with tobacco tissue cultures. Physiol. Plant. 15: 473–497.

    Google Scholar 

  • Nelson N. 1944. A photometric adaptation of Somogyi method for the determination of glucose. J. Biol. Chem. 153: 375–380.

    Google Scholar 

  • Ozbingol N., Corbineau F. and Come D. 1998. Response of tomato seeds to osmoconditioning as related to temperature and oxygen. Seed Sci. Res. 8: 377–384.

    Google Scholar 

  • Passam H.C. and Kakouriotis D. 1994. The effects of osmoconditioning on the germination, emergence and early plant growth of cucumber under saline conditions. Scientia-Horticulturae 57: 233–240.

    Google Scholar 

  • Ribaut J.M. and Pilet P.E. 1991. Effect of water stress on growth, osmotic potential and abscisic acid content of maize roots. Physiol Plant 81: 156–162.

    Google Scholar 

  • Ribaut J.M. and Pilet P.E. 1994. Water stress and indole-3-yl-acetic acid content of maize roots. Planta 193: 502–507.

    Google Scholar 

  • Roy D., Basu N., Mark R. and Banerjee S.K. 1995. Changes in levels for endogenous gibberellin and abscisic acid in salt sensitive germinating rice by NaCl salinity. Plant Physiol Biochem. 22: 200–202.

    Google Scholar 

  • Spyropoulos C.G. 1982. Control of sucrose metabolism in polyethylene glycol-stressed carob (Ceratonia siliqua L.) young seedlings. The role of sucrose. J. Exp. Bot. 33: 1210–1219.

    Google Scholar 

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Correspondence to Narinder Kaur.

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Kaur, S., Gupta, A.K. & Kaur, N. Effect of osmo- and hydropriming of chickpea seeds on seedling growth and carbohydrate metabolism under water deficit stress. Plant Growth Regulation 37, 17–22 (2002). https://doi.org/10.1023/A:1020310008830

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