Abstract
Spinach and pea plants were grown in hydroponic culture with nitrate orammonium salts as the nitrogen source. Dry matter accumulation andphotosynthetic rate declined in spinach plants fed with ammonium salts, whereasthey did not change in pea plants compared with nitrate-fed plants. Measurementof organic nitrogen and free amino acid content showed that ammonium ions wereassimilated in shoots in spinach plants and in roots in pea plants. Ammoniumionnutrition led to a decline in starch content in both species. Organic acidsincreased in roots of pea plants fed with ammonium ions whereas they declinedinspinach plants. In both species ammonium ions increased root respiration ratebut the contribution of both routes (cytochromic and alternative pathway) tothis increase was different depending on the species. In spinach plants,ammonium ions increased the cytochromic path and decreased the alternativepathway, whereas in pea plants both routes were stimulated mainly through thealternative pathway. The differences in the sensitivity to ammonium ionsbetweenboth species are discussed in terms of differences in the availability of Cskeletons and energy, which could be due in part to differences in the capacityto stimulate the alternative pathway.
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References
AOAC 1990. Official Methods of Analysis. Association of Official Analytical Chemistry, pp. 127–129.
Arnozis P.A., Nelemans J.A. and Findenegg G.R. 1988. Phosphoenolpyruvate carboxylase activity in plants grown with either NO-3 or NH+4 as inorganic nitrogen source. J. Plant Physiol. 132: 23–27.
Barker A.V., Volk R.J. and Jackson W.A. 1966. Growth and nitrogen distribution patterns in bean plants Phaseolus vulgaris L. Subjected to ammonium nutrition: I Effects of carbonates and acidity control. Soil Sci. Amer. Proc. 30: 228–232.
Barker A.V. and Mills H.A. 1980. Ammonium and nitrate nutrition of horticultural crops. Hort. Rev. 2: 395–423.
Barneix A.J., Breteler H., Siebe C. and Van de Geijn S.C. 1984. Gas and ion exchanges in wheat roots after nitrogen supply. Physiol. Plant 61: 357–362.
Blacquière T. 1987. Ammonium and nitrate nutrition in Plantago lanceolata L. and Plantago major L. ssp. major. II. Efficiency of root respiration and growth. Comparison of measured and theorical values of growth respiration. Plant Physiol. Biochem. 25: 775–785.
Blacquière T., Hofstra R. and Stulen I. 1987. Ammonium and nitrate nutrition in Plantago lanceolata L. and Plantago major L. ssp. major. I. Aspects of growth, chemical composition and root respiration. Plant Soil 104: 129–141.
Bloom A.J., Sukrapanna S.S. and Warner R.L. 1992. Root respiration associated with ammonium and nitrate absorption and assimilation by barley. Plant Physiol. 99: 1294–1301.
Bruning-Fann C. and Kaneene J.B. 1993. The effects of nitrate, nitrite and N-nitroso compounds on animal health. Vet. Hum. Toxicol. 35: 237–253.
Claussen W. and Lenz F. 1999. Effect of ammonium or nitrate nutrition on net photosynthesis, growth, and activity of the enzymes nitrate reductase and glutamine synthetase in blueberry, raspberry and strawberry. Plant Soil 208: 95–102.
Cox W.J. and Reisenauer H.M. 1973. Growth and ion uptake by wheat supplied nitrogen as nitrate or ammonium or both. Plant Soil 38: 363–380.
Chaillou S., Rideout J.W., Raper C.D. and Morot-Gaudry J.F. 1994. Responses of soybean to ammonium and nitrate supplied in combination to the whole root system or separately in a splitroot system. Physiol. Plant 90: 259–268.
Frechilla S., Gonzalez E.M., Royuela M., Minchin F.R., Aparicio-Tejo P.M. and Arrese-Igor C. 2000. Source of nitrogen nutrition (nitrogen fixation or nitrate assimilation) is a major factor involved in pea response to moderate water stress. J. Plant Physiol. 157: 609–617.
Gangolli S.D., Van Den Brandt P.A., Feron V.J., Jan-Zowsky C., Koeman J.H., Speijer G.J.A. et al. 1994. Nitrate, nitrite and N-nitroso compounds. Eur. J. Pharm., Envir. Tox. Pharm. Section 292: 1–38.
Haynes R.J. and Goh K.M. 1978. Ammonium and nitrate nutrition of plants. Biol. Rev. 53: 465–510.
Hoefnagel M.H.N., Rich P.R., Zhang Q. and Wiskich J.T. 1997. Substrate kinetics of the plant mitochondrial alternative oxidase and the effects of pyruvate. Plant Physiol. 115: 1145–1153.
Lasa B., Frechilla S., Lamsfu C. and Aparicio-Tejo P.M. 2001. The sensitivity to ammonium nutrition is related to nitrogen accumulation. Sci. Hort.688: 131–162.
Peuke A.D. and Jeschke W.D. 1993. The uptake and flow of C, N and ions between roots and shoots in Ricinus communis L. J. Exp. Bot. 44: 1167–1176.
Purvis A.C. and Shewfelt R.L. 1993. Does the alternative pathway ameliorate chilling injury in sensitive plant tissues? Physiol. Plant 88: 712–718.
Rigaud J. and Puppo A. 1975. Indole-3-acetic catabolism by soybean bacteroid. J. Gen. Microbiol. 88: 223–228.
Santamaria P., Elia A., Papa G. and Serio F. 1998. Nitrate and ammonium nutrition in chicory and rocket salad plants. J. Plant Nutr. 21: 1179–1789.
Turpin D.H., Elrifi I.R., Birch D.G., Weger H.G. and Holmes J.J. 1988. Interactions between photosynthesis, respiration, and nitrogen assimilation in microalgae. Can. J. Bot. 66: 2083–2097.
Wagner A.M. and Krab K. 1995. The alternative respiration pathway in plants: Role and regulation. Physiol. Plant 95: 318–325.
Weger H.G., Birch D.G., Elrifi I.R. and Turpin D.H. 1988. Ammonium assimilation requires mitochondrial respiration in the light. A study with the green alga Selenastrum minutum. Plant Physiol. 86: 688–692.
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Lasa, B., Frechilla, S., Aparicio-Tejo, P.M. et al. Alternative pathway respiration is associated with ammonium ion sensitivity in spinach and pea plants. Plant Growth Regulation 37, 49–55 (2002). https://doi.org/10.1023/A:1020312806239
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DOI: https://doi.org/10.1023/A:1020312806239