Abstract
Tomato (Lycopersicon esculentum L., cv. Sibirskii skorospelyi) and cucumber (Cucumis sativus L., cv. Konkurent) plants were grown in a soil culture in a greenhouse at an average daily temperature of 20°C and ambient illumination until the development of five and eight true leaves, respectively. During the subsequent three days, some plants were kept in a climatic chamber at 6°C in the light, whereas other plants remained in a greenhouse (control). The cold-resistance of cucumber leaves and roots, as assayed from the electrolyte leakage, was reduced after cold exposure stronger than cold-resistance of tomato organs. The ratio photosynthesis/dark respiration was lower in cucumber than in tomato leaves at all measurement temperatures. The concentrations of sugars (sucrose + glucose + fructose) increased in chilled tomato roots but decreased in cucumber roots. Cold exposure changed the activities of various invertase forms (soluble and insoluble acidic and alkaline invertases). The total invertase activity and the ratio of mono- to disaccharides increased. The lower cucumber cold-resistance is related to the higher sensitivity of its photosynthetic apparatus to chilling and, as a consequence, insufficient root supply with sugars.
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REFERENCES
Tumanov, I.I., Fiziologiya zakalivaniya i morozostoikosti rastenii (Physiology of Hardening and Frost-Resistance of Plants), Moscow: Nauka, 1979.
Murelli, C., Rizza, F., Albini, F.M., Dulio, A., Terzi, V., and Cattivelli, L., Metabolic Changes Associated with Cold-Acclimation in Contrasting Cultivars of Barley, Physiol. Plant., 1995, vol. 94, pp. 87-93.
Sasaki, H., Ishimura, K., and Oda, M., Changes in Sugar Content during Cold Acclimation and Deacclimation of Cabbage, Ann. Bot., 1996, vol. 78, pp. 365-369.
Travert, S., Valerio, L., Fouraste, I., Boudet, A.M., and Teulieres, C., Enrichment in Specific Soluble Sugars of Two Eucalyptus Cell-Suspension Cultures by Various Treatments Enhances Their Frost Tolerance via a Non-colligative Mechanism, Plant Physiol., 1997, vol. 114, pp. 1433-1442.
Sauter, J.J., Wisniewski, M., and Witt, W., Interrelationship between Ultrastructure, Sugar Levels, and Frost Hardiness of Ray Parenchyma Cells during Frost Acclimation and Deacclimation in Poplar (Populus canadensis Moench “robusta”) Wood, J. Plant Physiol., 1998, vol. 149, pp. 451-461.
Zabotina, O.A., Ayupova, D.A., Larskaya, I.A., Nikolaeva, O.G., Petrovicheva, G.A., and Zabotin, A.I., Physiologically Active Oligosaccharides Accumulating in the Roots of Winter Wheat during Adaptation to Low Temperature, Fiziol. Rast. (Moscow), 1998, vol. 45, pp. 262-267 (Russ. J. Plant Physiol., Engl. Transl.).
Uemura, M. and Steponkus, P.L., Alterations in the Incidence of Freeze-Induced Lesions of Arabidopsis Protoplasts by Artificial Manipulation of Intracellular Sugar Content, Plant Cell Physiol., 1998, vol. 39, p. 140.
Trunova, N.I., Klimov, S.V., and Astakhova, N.V., The Role of Photosynthesis in Chilling Resistance of Tomato and Cucumber, Hortic. Veget. Growing, 1998, vol. 17, pp. 236-244.
Klimov, S.V., Popov, V.N., and Trunova, T.I., The Relationship between the Cold Tolerance of Tomato and Cucumber Organs and Photosynthesis, Fiziol. Rast. (Moscow), 2000, vol. 47, pp. 501-506 (Russ. J. Plant Physiol., Engl. Transl.).
Klimov, S.V., Astakhova, N.V., and Trunova, T.I., Changes in Photosynthesis, Dark Respiration Rates and Photosynthetic Carbon Partitioning in Winter Rye and Wheat Seedlings during Cold Hardening, J. Plant Physiol., 1999, vol. 155, pp. 734-739.
Turkina, M.V. and Sokolova, S.V., Methods for Determination of Monosaccharides and Oligosaccharides, Biokhimicheskie metody v fiziologii rastenii (Biochemical Methods in Plant Physiology), Pavlinova, O.A., Ed., Moscow: Nauka, 1971, pp. 7-34.
Drozdov, S.N., Sycheva, Z.F., Budykina, N.P., and Kurets, V.K., Ekologofiziologicheskie aspekty ustoichivosti rastenii k zamorozkam (Ecological and Physiological Aspects of Plant Resistance to Light Frosts), Leningrad: Nauka, 1977.
Klimov, S.V. and Trunova, T.I., An Increased Ratio of Photosynthesis to Respiration at Low Temperatures Is a Prerequisite for Cold Hardening of Winter Cereals, Mrozoodpornosc, Holubowicz, T., Ed., Poznan: Polska Akad. Nauk, 1999, pp. 61-67.
Artuso, A., Guidi, L., Soldatini, G.F., Pardossi, A., and Tognoni, F., The Influence of Chilling on Photosynthesis and Activities of Some Enzymes of Sucrose Metabolism in Lycopersicon esculentum Mill., Acta Physiol. Plant., 2000, vol. 22, pp. 95-101.
Savitch, L.V., Harney, T., and Huner, N.P.A., Sucrose Metabolism in Spring and Winter Wheat in Response to High Irradiance, Cold Stress and Cold Acclimation, Physiol. Plant., 2000, vol. 108, pp. 270-278.
Kolupaev, Yu.E. and Trunova, T.I., Hypothermia and Salt Stress Influence on Invertase Activity and Carbohydrate Content in Wheat Coleoptyles, Fiziol. Rast. (Moscow), 1994, vol. 41, pp. 552-557 (Russ. J. Plant Physiol., Engl. Transl.).
Livingston, D.P. III and Henson, C.A., Apoplastic Sugars, Fructans, Fructan Exohydrolase, and Invertase in Winter Oat: Responses to Second-Phase Cold Hardening, Plant Physiol., 1998, vol. 116, pp. 403-408.
Sturm, A., Invertases: Primary Structures, Functions and Roles in Plant Development and Sucrose Partitioning, Plant Physiol., 1999, vol. 121, pp. 1-7.
Zauralov, O.A., Lukatkin, A.S., and Sharkaeva, E.Sh., Intracellular pH of Maize Leaf Tissues under Different Dynamics of Chilling, Izv. RAN, Ser. Biol., 1997, no. 1, pp. 96-99.
Zauralov, O.A. and Lukatkin, A.S., Intracellular pH of Chilling-Sensitive Leaves under Low Temperatures, Fiziol. Biokhim. Kul't. Rast., 1988, vol. 20, pp. 586-592.
Suleimanov, I.G., Strukturno-fizicheskie svoistva protoplazmy i ee komponentov v svyazi s problemoi morozo-ustoichivosti kul'turnykh rastenii (Structural and Physical Properties of the Protoplasm and Its Components as Related to the Frost Tolerance of Cultivated Plants), Kazan: Izd-vo Kazansk. Univ., 1964.
Yoshida, S., Low Temperature-Induced Cytoplasmic Acidosis in Cultured Mung Bean (Vigna radiata (L.) Wilczek) Cells, Plant Physiol., 1994, vol. 104, pp. 1131-1138.
Klimov, S.V., Trunova, T.I., and Mokronosov, A.T., Mechanism of Plant Adaptation to Unfavorable Environmental Conditions by Changing Source-Sink Relations, Fiziol. Rast. (Moscow), 1990, vol. 37, pp. 1024-1035 (Sov. Plant Physiol., Engl.Transl.).
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Klimov, S.V., Popov, V.N., Dubinina, I.M. et al. The Decreased Cold-Resistance of Chilling-Sensitive Plants Is Related to Suppressed CO2 Assimilation in Leaves and Sugar Accumulation in Roots. Russian Journal of Plant Physiology 49, 776–781 (2002). https://doi.org/10.1023/A:1020913612405
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DOI: https://doi.org/10.1023/A:1020913612405