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Development of Linum flavum hairy root cultures for production of coniferin

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Abstract

Linum flavum hairy roots were initiated from leaf discs using Agrobacterium rhizogenes strains LBA9402 and TR105 though two other strains, 15834 and A4, were relatively ineffective for induction. Significant variation in coniferin accumulation was observed between hairy root lines originating from different L. flavum seedlings and/or A. rhizogenes strains. Coniferin reached 58 mg g−1 dry wt by culturing the roots in Linsmaier and Skoog (LS) medium with 2,4-dichlorophenoxyacetic acid and naphthaleneacetic acid as growth regulators.

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Authors and Affiliations

  1. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Sydney, NSW, 2052, Australia

    Han-wei Lin & Pauline M. Doran

  2. Cooperative Research Centre (CRC) for Bioproducts, Tridan Limited–Albright & Wilson (Australia) Limited, P.O. Box 20, Yarraville, VIC, 3013, Australia

    Kian H. Kwok

Authors
  1. Han-wei Lin
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  2. Kian H. Kwok
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  3. Pauline M. Doran
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Correspondence to Pauline M. Doran.

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Lin, Hw., Kwok, K.H. & Doran, P.M. Development of Linum flavum hairy root cultures for production of coniferin. Biotechnology Letters 25, 521–525 (2003). https://doi.org/10.1023/A:1022821600283

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  • DOI: https://doi.org/10.1023/A:1022821600283

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