biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:589-594, 2003 | DOI: 10.1023/A:1024827931134

Alleviation of NaCl Stress by Pretreatment with Phytohormones in Vigna radiata

N. Chakrabarti1, S. Mukherji2
1 Plant Physiology Laboratory, Department of Botany, University of Calcutta, Calcutta-, India
2 Plant Physiology Laboratory, Department of Botany, University of Calcutta, Calcutta-, India

Efficiency of pretreatment as foliar spray of indole-3-acetic acid, gibberellic acid and kinetin, each ranging from 0.1 to 10.0 μM concentration, in restoring the metabolic alterations imposed by NaCl salinity was investigated in Vigna radiata (L.) Wilczek. Glycolate oxidase, superoxide dismutase, catalase and peroxidase activities increased under stress in leaves and roots also. Malondialdehyde content and total peroxide content also increased under stress. All the three hormones used were able to overcome to variable extents the adverse effects of stress imposed by NaCl to these parameters.

Keywords: catalase; glycolate oxidase; malondialdehyde; peroxidase; peroxide; superoxide dismutase
Subjects: auxins; catalase; cytokinins; gibberellins; glycolate oxidase; malondialdehyde; mung bean, salt stress, growth regulators; peroxidase; salt stress alleviation, growth regulators; superoxide dismutase; Vigna radiata

Prepublished online: May 1, 2003; Published: December 1, 2003  Show citation

ACS AIP APA ASA Harvard Chicago IEEE ISO690 MLA NLM Turabian Vancouver
Chakrabarti, N., & Mukherji, S. (2003). Alleviation of NaCl Stress by Pretreatment with Phytohormones in Vigna radiata. Biologia plantarum46(4), 589-594. doi: 10.1023/A:1024827931134
Download citation

References

  1. Abd-Alla, M.H., Vuong, T.D., Harper, J.E.: Genotypic differences in dinitrogen fixation response to NaCl stress in intact and grafted soybean.-Crop Sci. 38: 72-77, 1998. Go to original source...
  2. Chance, B., Maehly, A.C.: Assay of catalases and peroxidases.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 2. Pp. 764-775. Academic Press, New York 1955. Go to original source...
  3. Choudhuri, M.A.: Free radicals and leaf senescence-a review.-Plant Physiol. Biochem. 15: 18-29, 1988.
  4. Dhindsa, R.S.: Inhibition of protein synthesis by products of lipid peroxidation.-Phytochemistry 21: 309-313, 1982. Go to original source...
  5. Gasper, T., Lacoppe, J.: The effect of CCC and AMO-1618 on growth, catalase, peroxidase, IAA-oxidase activity of young barley seedlings.-Physiol. Plant. 2: 1104-1109, 1968. Go to original source...
  6. Giridara Kumar, S., Madhusudhan, K.V., Sreenivasulu, N., Sudhakar, C.: Stress responses in two genotypes of mulberry (Morus alba L.) under NaCl salinity.-Indian J. exp. Biol. 38: 192-195, 2000.
  7. Heath, R.L., Packer, L.: Photoperoxidation in isolated chloroplasts. I. Kinetics and stoichiometry of fatty acid peroxidation.-Arch. Biochem. Biophys. 125: 189-198, 1968. Go to original source...
  8. Kalir, A., Omri, G., Poljakoff-Mayber, A.: Peroxidase and catalase activity in leaves of Halimione portulacoides exposed to salinity.-Physiol. Plant. 62: 238-244, 1984. Go to original source...
  9. Konze, J.R., Elstner, E.F.: Ethane and ethylene formation by mitochondria as indication of aerobic lipid degradation in response to wounding of plant tissue.-Biochem. biophys. Acta. 528: 213-221, 1978. Go to original source...
  10. Letham, D.S.: The cytokinins.-In: Letham, D.S., Goodwin, P.B., Higgins, T.J.V. (ed.): Phytohormones and Related Compounds. A Comprehensive Treatise. Vol. 1. Pp. 205-263. Elsevier/North-Holland Biomedical Press, Amsterdam 1978.
  11. Lowry, O.H., Rosebrough, N.J., Farr, A.L., Randall, R.J.: Protein measurement with the Folin phenol reagent.-J. biol. Chem. 193: 265-275, 1951. Go to original source...
  12. Marshall, M.J., Worsfold, M.: Superoxide dismutase: a direct, continuous linear assay using the oxygen electrode.-Anal. Biochem. 86: 561-573, 1978. Go to original source...
  13. Miszalski, Z., Slesak, I., Niewiadomska, E., Baczek-Kwinta, R., Lüttge, U., Ratajczak, R.: Subcellular localization and stress responses of superoxide dismutase isoforms from leaves in the C3-CAM intermediate halophyte Mesembryanthemum crystallinum L.-Plant Cell Environ. 21: 169-179, 1998. Go to original source...
  14. Piqueras, A., Hernandez, J.A., Olmos, E., Hellin, E., Sevilla, F.: Changes in antioxidant enzymes and organic solutes associated with adaptation of Citrus cells to salt stress.-Plant Cell Tissue Organ Cult. 45: 53-60, 1996. Go to original source...
  15. Satakopan, V.N., Devi, N.A., Srinivasan, R.: Inhibition of peroxidase during germination of Arachis hypogaea by combined salinities of NaCl and CaCl2.-Indian J. Plant Physiol. 33: 85-86, 1990.
  16. Sheokand, S., Dhandi, S., Swaraj, K.: Studies on nodule functioning and hydrogen peroxide scavenging enzymes under salt stress in chickpea nodules.-Plant Physiol. Biochem. 33: 561-566, 1995.
  17. Subhashini, K., Reddy, G.M.: Effect of salt stress on enzyme activities in callus cultures of tolerant and susceptible rice cultivars.-Indian J. exp. Biol. 28: 277-279, 1990.
  18. Thurman, R.G., Ley, H.G., Scholz, R.: Hepatic microsomal ethanol oxidation hydrogen peroxide formation and the role of catalase.-Eur. J. Biochem. 25: 420-430, 1972. Go to original source...
  19. Venkatesan, A., Chellappan, K.P.: Salinity effect on the activities of certain antioxidant enzymes in Ipomoea pescaprae sweet, a halophyte.-Indian J. Plant Physiol. 4: 40-42, 1999.
  20. Zelitch, I.: Glycolic acid oxidase.-In: Colowick, S.P., Kaplan, N.O. (ed.): Methods in Enzymology. Vol. 1. Pp. 528-532. Academic Press, New York 1953.