biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 43:153-155, 2000 | DOI: 10.1023/A:1026535920714

Primer Design and Optimization for RAPD Analysis of Nepenthes

S.H. Lim1, D.C.Y. Phua1, H.T.W. Tan1
1 Department of Biological Sciences, The National University of Singapore, Singapore, Republic of Singapore e-mail

Primers with higher G+C content produced better random amplified polymorphic DNA (RAPD) profiles in Nepenthes. The occurrence of clustered G's and C's in the center of the primer seemed also to influence the banding patterns. It was also observed that for certain polymerases, the use of different buffers other than that recommended by the manufacturer provided a better amplification profile for Nepenthes.

Keywords: GC content; PCR buffer; RAPD markers
Subjects: GC content, primer design for RAPD analysis; Nepenthes; polymerase chain reaction, PCR; random amplified polymorphic DNA, RAPD markers

Published: March 1, 2000  Show citation

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Lim, S.H., Phua, D.C.Y., & Tan, H.T.W. (2000). Primer Design and Optimization for RAPD Analysis of Nepenthes. Biologia plantarum, 43(1), 153-155. doi: 10.1023/A:1026535920714
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