biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:153-156, 2003 | DOI: 10.1023/A:1027365822769

Mutation in Flower Colour and Shape of Chrysanthemum morifolium Induced by γ-Radiation

P. Misra1, S.K. Datta1, D. Chakrabarty1
1 Floriculture Section, National Botanical Research Institute, Lucknow, India

Flowers of Chrysanthemum morifolium Ramat cv. Lalima were greyed red and florets were flat spoon shaped. Ray florets after inoculation on the Murashige and Skoog's medium supplemented with 1.07 μM α-naphthaleneacetic acid and 8.87 μM benzyladenine were irradiated with γ-radiation (0.5 Gy and 1 Gy). All the regenerated shoots either from control or from γ-irradiated florets were isolated, rooted and transplanted in the field after hardening. Two mutants were obtained in the γ-irradiated plants (0.5 Gy). Both the mutants were yellow coloured but one having flat spoon shaped ray florets similar to the original cultivar, while the other having tubular florets. Both the mutants were propagated vegetatively and have produced true-to-type flowers.

Keywords: chimera; in vitro mutagenesis; ray florets
Subjects: auxins; Chrysanthemum morifolium; flower, ray florets; in vitro culture, mutagenesis; mutation induced by γ-radiation, flower colour; radiation, gamma, in vitro mutagenesis

Published: July 1, 2003  Show citation

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Misra, P., Datta, S.K., & Chakrabarty, D. (2003). Mutation in Flower Colour and Shape of Chrysanthemum morifolium Induced by γ-Radiation. Biologia plantarum, 46(1), 153-156. doi: 10.1023/A:1027365822769
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    References

    1. Al-Safadi, B., Ayyoubi, Z., Jawdat, D.: The effect of gamma irradiation on potato microtuber production in vitro.-Plant Cell Tissue Organ Cult. 61: 183-187, 2000. Go to original source...
    2. Broertjes, C., van Harten, A.M.: Applied mutation breeding for vegetatively propagated crops.-Elsevier, Amsterdam 1988. Go to original source...
    3. Chakrabarty, D., Mandal, A.K.A., Datta, S.K.: Management of chimera through direct shoot regeneration from florets of chrysanthemum (Chrysanthemum morifolium Ramat.).-J. Hort. Sci. Biotechnol. 74: 293-296, 1999. Go to original source...
    4. Chakravarty, B., Sen, S.: Enhancement of regeneration potential and variability by γ-irradiation in cultured cells of Scilla indica.-Biol. Plant. 44: 189-193, 2001. Go to original source...
    5. Datta, S.K.: Chrysanthemum cultivars evolved by induced mutations at National Botanical Research Institute, Lucknow.-Chrysanthemum 44: 72-75, 1988.
    6. Datta, S.K.: Chrysanthemum germplasm at NBRI and search for novel genes.-Appl. bot. Abstr. 28: 45-72, 1998.
    7. Datta, S.K., Chakrabarty, D., Mandal, A.K.A.: Gamma ray induced genetic manipulations in Dendranthema grandiflorum and their management through tissue culture.-Plant Breed. 120: 91-92, 2001. Go to original source...
    8. Malaure, R.S., Barclay, G., Power, J.B. Davey, M.R.: The production of novel plants from florets of Chrysanthemum morifolium using tissue culture. I. Shoot regeneration from ray florets and somaclonal variation exhibited by the regenerated plants.-J. Plant Physiol. 139: 8-13, 1991. Go to original source...
    9. Mandal, A.K.A., Datta, S.K., Chakrabarty, D.: In vitro development of novel flower colour through management of induced chimera.-Euphytica 114: 9-12, 2000. Go to original source...
    10. Murashige, T., Skoog, F.: A revised medium for rapid growth and bio-assays with tobacco tissue culture.-Physiol. Plant. 15: 473-497, 1962. Go to original source...

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