Abstract
The genus Pectinatus is currently composed by two species, Pectinatus cerevisiiphilus and Pectinatus frisingensis, both asociated with beer spoilage. This study describes a novel isolate (strain B6) retrieved from a wastewater treatment plant collecting residues from a large number of wineries. Based on similarity analysis of 16S rRNA gene sequences, strain B6 belongs to the genus Pectinatus. Strain B6 is a strict anaerobe like other Pectinatus species and it presents non-motile, coccoid cells showing a slight oval shape. Strain B6 shows marked physiological differences with other Pectinatus species both in fatty acid composition and carbon source utilization. The most abundant fatty acids found in strain B6 were 18:1 (42.8%) and 16:0 (18.3%) representing a total of over 61% of fatty acids in this microorganism while these fatty acids represented 41.3% in P. cerevisiiphilus T and 2.4% in P. frisingensis T of their total. Fatty acid 15:0 was not significant in strain B6 and represented 28.6% and 13.3% for P. cerevisiiphilus T and P. frisingensis T, respectively. Strain B6 showed a faster growth rate and higher optimum temperature than its relatives P. cerevisiiphilus and P. frisingensis. Strain B6, P. cerevisiiphilus and P. frisingensis could be clearly differentiated by acid production tests from substrates such as esculine and gluconate, and the lack of acid production from rhamnose and fucose among others. G+C mol% content in strain B6 is 36.5%. Based on genotypic and phenotypic differences, strain B6 is proposed as a novel Pectinatus species, P. portalensis nov. sp. Both strain B6 and the two described species of Pectinatus grow on beers and wines. These results provide insights about the origin and reservoirs of Pectinatus species and spoiling alcoholic beverages.
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References
Altschul S.F., Gish W., Miller W., Myers E.W. and Lipman D.J. 1990. Basic local alignment search tool. J. Biol. Mol. 215: 403-410.
Chowdhury I., Watier D. and Hornez J.P. 1995. Variability in sur-vival of Pectinatus cerevisiiphilus, strictly anaerobic bacteria, under different oxygen conditions. Anaerobe 1: 151-156.
De Ley J., Cattoir H. and Reynaerts A. 1970. The quantitative measurement of DNA hybridization from renaturation rates. Eur. J. Biochem. 12: 133-142.
Dürr P. 1983. Luftkeimindikation bierschädlicher Bakterien: Neue Methode mittels Luftkeimsammelgerät und Luftkeimindikator. Brauwelt 123: 1652-1655.
Gerhardt P., Murray R.G.E., Wood W.A. and Krieg N.R. 1994. Methods for General and Molecular Bacteriology. American So-ciety for Microbiology, Washington, DC, USA.
Gonzalez J.M., Kato C. and Horikoshi K. 1995. Thermococcus peptonophilus sp. nov., a fast-growing, extremely thermophilic archaebacterium isolated from deep-sea hydrothermal vents. Arch. Microbiol. 164: 159-164.
González J.M. and C. Saiz-Jimenez. 2002. A fluorimetric method for the estimation of G+C mol% content in microorganisms by thermal denaturation temperature. Environ. Microbiol. 4: 770-773.
Haikara A. 1985. Detection of Pectinatus contaminants in beer. J. Am. Soc. Brew. Chem. 43: 43-46.
Haikara A. and Helander I. 2001. Pectinatus, Megasphaera and Zymophilus. In: Dworkin M. (eds), The Prokaryotes, 3rd edition, release 3.5. Springer-Verlag, New York, New York, USA.
Hakalehto E. 2000. Characterization of Pectinatus cerevisiiphilus and P. frisingiensis surface components. Use of synthetic pep-tides in the detection of some Gram-negative bacteria. Natural and Environmental Sciences, Kuopio University Publications Kuopio, Finland 112: 70.
Helander I.M. and Haikara A. 1995. Cellular fatty and alkenyl residues in Megasphaera and Pectinatus species: contrasting profiles and detection of beer spoilage. Microbiology 141: 1131-1137.
Lee S.Y., Mabee M.S. and Jangaard N.O. 1978. Pectinatus, a new genus of the family Bacteroidaceae. Int. J. Syst. Bacteriol. 28: 582-594.
Lee S.Y. 1984. Genus XI Pectinatus.. In: Krieg N.R. and Holt J.G. (eds), Bergey's Manual of Systematic Bacteriology, Williams and Wilkins Baltimore, Maryland, USA, vol. 1, pp. 655-658.
Ludwig W., Strunk O., Klugbauer N., Weizenegger M., Neumaier J., Bachleitner M. and Schleifer K.H. 1998. Bacterial phylogeny based on comparative sequence analysis. Electrophoresis 19: 554-568.
Marmur J. 1961. A procedure for the isolation of deoxyribonucleic acid from microorganisms. J. Mol. Biol. 3: 208-218.
Moore L.V., Bourne D.M. and Moore W.E.C. 1994. Comparative distribution and taxonomic value of cellular fatty acids in thirty-247.three genera of anaerobic Gram-negative bacilli. Int. J. Syst. Bacteriol. 44: 338-347.
Roselló-Mora R. and Amann R. 2001. The species concept for prokaryotes. FEMS Microbiol Rev 25: 39-67.
Schleifer K.H., Leuteritz M., Weiss N., Ludwig W., Kirchof G. and Seidel-Rüfer H. 1990. Taxonomic study of anaerobic, gram-negative, rod-shaped bacteria from breweries: Emended descrip-tion of Pectinatus cerevisiiphilus and description of Pectinatus frisingensis sp. nov., Selenomonas lacticifex sp. nov., Zymophi-lus raffýnosivorans gen. nov., sp. nov., and Zymophilus paucivorans sp. nov. Int. J. Syst. Bacteriol. 40: 19-27.
Soberka R. and Warzecha A. 1986. Influence de certains facteurs sur le taux d'oxygène dissous au cours de la fabrication de la bière. Bios. 17: 31-40.
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Gonzalez, J.M., Jurado, V., Laiz, L. et al. Pectinatus portalensis nov. sp., a relatively fast-growing, coccoidal, novel Pectinatus species isolated from a wastewater treatment plant. Antonie Van Leeuwenhoek 86, 241–247 (2004). https://doi.org/10.1023/B:ANTO.0000047931.55259.33
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DOI: https://doi.org/10.1023/B:ANTO.0000047931.55259.33