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Cloning and Nucleotide Sequence of the Endoinulinase-Encoding Gene, inu2, from Aspergillus ficuum

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Abstract

A 2.3 kb DNA fragment that contains a gene encoding endoinulinase, inu2, from Aspergillus ficuum ATCC 16882 was isolated and analyzed. It includes an open reading frame of 1,551 bp, coding for a polypeptide with calculated molecular weight of 55,790 Da, including a putative signal peptide of 22 amino acids. Alignment of amino acid sequences revealed 73.3% identity and 93.9% similarity between A. ficuum and Penicillium purpurogenum endoinulinase.

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Authors and Affiliations

  1. Faculty of Biological Sciences and Institute for Molecular Biology and Genetics, Chonbuk National University, Chonju, 561-756, Korea

    T.B. Uhm, K.-S. Chae, D.W. Lee & H-S. Kim

  2. Laboratoire de Genetique Moleculaire, Facultes Universitaires Notre-Dame de la Pair, Rue de Bruxelles, 61, B-5000, Namur, Belgium

    J-P. Cassart & J. Vandenhaute

Authors
  1. T.B. Uhm
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  2. K.-S. Chae
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  3. D.W. Lee
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  4. H-S. Kim
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  5. J-P. Cassart
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  6. J. Vandenhaute
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Uhm, T., Chae, KS., Lee, D. et al. Cloning and Nucleotide Sequence of the Endoinulinase-Encoding Gene, inu2, from Aspergillus ficuum . Biotechnology Letters 20, 809–812 (1998). https://doi.org/10.1023/B:BILE.0000015928.45007.30

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  • DOI: https://doi.org/10.1023/B:BILE.0000015928.45007.30

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