Abstract
The kinetics of enzymatic hydrolysis of rice bran oil in isooctane by immobilized Candida rugosa lipase in a batch reactor showed competitive inhibition by isooctane with a dissociation constant, KI, of 0.92 m. Continuous hydrolysis of rice bran oil was performed in recycling, packed bed reactor with 4352 U of immobilized lipase; the optimum recycle ratio was 9 and the operational half-life was 360 h without isooctane but 288 h with 25% (v/v) isooctane in rice bran oil.
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Murty, V.R., Bhat, J. & Muniswaran, P. Hydrolysis of rice bran oil using an immobilized lipase from Candida rugosa in isooctane. Biotechnology Letters 26, 563–567 (2004). https://doi.org/10.1023/B:BILE.0000021956.33855.11
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DOI: https://doi.org/10.1023/B:BILE.0000021956.33855.11