biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:429-432, 2003 | DOI: 10.1023/B:BIOP.0000023888.82993.b1

Somatic Embryogenesis, Plantlet Regeneration and Micropropagation of Cultivars and F1 Hybrids of Manihot esculenta

M.N. Ogburia1
1 Department of Crop/Soil Science & Forestry, Faculty of Agriculture, Rivers State University of Science and Technology, Port Harcourt, Nigeria

Explants of four F1 hybrids (OMR 36-41/1, OMR 36-41/2, OMR 36-41/4 and OMR 36-41/5) and two cultivars (Rayong 1 and Rayong 60) of cassava (Manihot esculenta Crantz) were subjected to different combinations of 2,4-dichlorophenoxyacetic acid (2,4-D), 1-naphthaleneacetic acid (NAA), kinetin (KIN) and N6-benzylaminopurine (BAP) to induce somatic embryogenesis, organogenesis and micropropagation. Shoot apices of the F1 hybrids exhibited higher frequency (62 - 74 %) of proliferation of somatic embryos than the cultivars (21 - 43 %) in Murashige and Skoog basal medium supplemented with 8 mg dm-3 2,4-D and 0.5 mg dm-3 NAA. Nodal explants of regenerated plantlets were rapidly micropropagated with 90 % efficiency on a medium containing 0.1 mg dm-3 NAA and 0.05 mg dm-3 BAP irrespective of explant source.

Keywords: 2,4-dichlorophenoxyacetic acid; in vitro growth; 1-naphthaleneacetic acid; organogenesis; phytohormones
Subjects: auxins; cassava, micropropagation; cultivar and genotype differences, micropropagation; cytokinins; 2,4-dichlorophenoxyacetic acid; in vitro culture, micropropagation; in vitro culture, nutrient media modification; in vitro culture, somatic embryogenegis; Manihot esculenta

Published: November 1, 2003  Show citation

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Ogburia, M.N. (2003). Somatic Embryogenesis, Plantlet Regeneration and Micropropagation of Cultivars and F1 Hybrids of Manihot esculenta. Biologia plantarum, 46(3), 429-432. doi: 10.1023/B:BIOP.0000023888.82993.b1
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