biologia plantarum

International journal on Plant Life established by Bohumil Němec in 1959

Biologia plantarum 46:527-533, 2003 | DOI: 10.1023/B:BIOP.0000041057.11390.58

Activities of Antioxidant Systems During Germination of Chenopodium rubrum Seeds

T. Dučić1, I. Lirić-Rajlić1, A. Mitrović1, K. Radotić1
1 Center for Multidisciplinary Studies, University of Belgrade, 29. Novembar 142, Belgrade, Serbia and Montenegro

The activities of superoxide-dismutase (SOD), catalase (CAT) and peroxidase (POD), and concentrations of glutathione and ascorbate have been studied during the first stages of germination in Chenopodium rubrum L. seeds. The highest CAT and SOD activity was found prior to radicle protrusion, while POD activity was maximal at the time of radicle protrusion and seedling development, new POD isozymes simultaneously appearing. The concentrations of total, reduced and oxidized glutathione showed similar changes during germination, the highest values being detected at the time of radicle protrusion. Ascorbic acid was present in the seeds in a detectable concentration only at the time preceding radicle protrusion, while its oxidized form dehydroascorbic acid was detected during the whole germination period studied. Gibberellic acid (GA3, 160 μM) had no effect on germination percentage, but in presence of GA3, SOD and CAT activity notably increased prior to radicle protrusion, and oxidized glutathione concentration decreased in further germination.

Keywords: ascorbic acid; catalase; gibberellic acid; glutathione; peroxidase; reactive oxygen species; superoxide-dismutase
Subjects: antioxidants; ascorbate, ascorbic acid; catalase; Chenopodium rubrum; dehydroascorbate; germination; gibberellins; glutathione, germination; peroxidases; protein concentration, germination; reactive oxygen species (ROS); superoxide dismutase

Published: December 1, 2003  Show citation

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Dučić, T., Lirić-Rajlić, I., Mitrović, A., & Radotić, K. (2003). Activities of Antioxidant Systems During Germination of Chenopodium rubrum Seeds. Biologia plantarum, 46(4), 527-533. doi: 10.1023/B:BIOP.0000041057.11390.58
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