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Immobilization of cell-associated enzymes by entrapment in polymethacrylamide beads

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Biotechnology Techniques

Abstract

Immobilization of E. coil, Aspergillus niger and Aspergillus japonicus was accomplished by entrapment in polymethacrylamide beads via a two-phase polymerization using soybean oil as the suspension medium. The immobilized E. coli cells containing penicillin G acylase possessed a higher activity than that reported in the literature. The mmobilized mycelia of Aspergillus sp. containing β-fructofuranosidase were effective for catalyzing the formation of fructooligosaccharides.

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Authors and Affiliations

  1. Department of Chemical Engineering, National Chung Cheng University, Chiayi, 621, Taiwan

    Chung-Jen Chiang, Liang-Tzung Hsiau & Wen-Chien Lee

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  1. Chung-Jen Chiang
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  2. Liang-Tzung Hsiau
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  3. Wen-Chien Lee
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Chiang, CJ., Hsiau, LT. & Lee, WC. Immobilization of cell-associated enzymes by entrapment in polymethacrylamide beads. Biotechnology Techniques 11, 121–125 (1997). https://doi.org/10.1023/B:BITE.0000034016.43050.22

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  • DOI: https://doi.org/10.1023/B:BITE.0000034016.43050.22

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