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Prophylactic action of melatonin against cyclophosphamide-induced oxidative stress in mice

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Abstract

The present study investigated the prophylactic influence of melatonin against cyclophosphamide-induced oxidative stress in mouse tissues. Lipid peroxidation, reduced glutathione (GSH), glutathione disulphide (GSSG), glutathione peroxidase (GSH-Px) and serum phosphatase levels were analyzed in brain, spleen liver, lungs, kidney and testes. Fifteen days oral administration with melatonin (0.1 mg/kg bw per day) before treatment checked the augmentation of the level of lipid peroxidation, blood GSSG and acid phosphatase caused by an acute treatment with a radiomimetic drug, cyclophosphamide (75 mg/kg bw). Cyclophosphamide-induced depletion in the level of GSH, GSH-Px and alkaline phosphatase was made up statistically significant by chronic melatonin administration given orally. The results indicate the antioxidative properties of melatonin resulting into its prophylactic property against the cyclophosphamide-induced biochemical alterations. The finding support the idea that melatonin is a potent free-radical scavenger and antioxidant.

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Authors and Affiliations

  1. Laboratory of Neurobiology and Aging, Department of Zoology, University of Rajasthan, Jaipur, 302 004, India

    K. Manda & A.L. Bhatia

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  1. K. Manda
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  2. A.L. Bhatia
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Correspondence to A.L. Bhatia.

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Manda, K., Bhatia, A. Prophylactic action of melatonin against cyclophosphamide-induced oxidative stress in mice. Cell Biol Toxicol 19, 367–372 (2003). https://doi.org/10.1023/B:CBTO.0000013342.17370.16

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