Skip to main content
SpringerLink
Log in

Decreased Total and Corrected Antioxidant Capacity in Patients with Inflammatory Bowel Disease

  • Published:
Digestive Diseases and Sciences Aims and scope Submit manuscript

Abstract

Oxidative stress and depletion of antioxidants may play a key role in the pathogenesis of inflammatory bowel disease (IBD)-related intestinal damage. A new automated assay for the determination of blood total antioxidant capacity (TAC), based on the crocin bleaching method, has been used for the measurement of TAC and corrected TAC (cTAC) in patients with ulcerative colitis (UC) and Crohn's disease (CD) in comparison to healthy controls (HC). Ninety-four patients with UC, 97 patients with CD, and 72 HC were included in this study. Serum TAC was measured in all patients and controls on an Olympus AU-600 chemistry analyzer using a TAC kit. cTAC was calculated from TAC after subtraction of the interactions due to endogenous uric acid, bilirubin and albumin. Mean serum TAC as well as cTAC levels were significantly lower in both UC and CD patients compared with HC (P<0.0001). Patients with active UC had no different TAC and cTAC compared to those with inactive disease. Patients with active CD had significantly lower mean TAC compared to those with inactive disease but cTAC was not different between the two phases of disease activity. Patients with proctitis had significantly higher TAC and cTAC compared to patients with left-sided colitis and total colitis. In CD patients no association between disease localization and these markers was found. TAC and cTAC are significantly reduced in IBD patients compared with controls irrespective of disease activity. The decreased antioxidant defenses may be a primary phenomenon severely compromising the mucosa and therefore increase susceptibility to oxidative tissue damage.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

REFERENCES

  1. Grisham MB: Oxidants and free radicals in inflammatory bowel disease. Lancet 24(344):859–861, 1994

    Google Scholar 

  2. Kruidenier L, Verspaget HW: Review article: Oxidative stress as a pathogenic factor in inflammatory bowel disease-Radicals or ridiculous? Aliment Pharmacol Ther 16:1997–2015, 2002

    Google Scholar 

  3. Kitahora T, Suzuki K, Asakura H, Yoshida T, Suematsu M, Watanabe M, Aiso S, Tsuchiya M: Active oxygen species generated by monocytes and polymorphonuclear cells in Crohn's disease. Dig Dis Sci 33:951–955, 1988

    Google Scholar 

  4. Keshavarzian A, Sedghi S, Kanofsky J, List T, Robinson C, Ibrahim C, Winship D: Excessive production of reactive oxygen metabolites by inflamed colon: Analysis by chemiluminescence probe. Gastroenterology 103:177–185, 1992

    Google Scholar 

  5. D'Odorico A, Bortolan S, Cardin R, D'Inca' R, Martines D, Ferronato A, Sturniolo GC: Reduced plasma antioxidant concentrations and increased oxidative DNA damage in inflammatory bowel disease. Scand J Gastroenterol 36:1289–1294, 2001

    Google Scholar 

  6. Kruidenier L, Kuiper I, Lamers CB, Verspaget HW: Intestinal ox-idative damage in inflammatory bowel disease: Semi-quantification, localization, and association with mucosal antioxidants. J Pathol 201:28–36, 2003

    Google Scholar 

  7. Genser D, Kang MH, Vogelsang H, Elmadfa I: Status of lipidsoluble antioxidants and TRAP in patients with Crohn's disease and healthy controls. Eur J Clin Nutr 53:675–679, 1999

    Google Scholar 

  8. Kruidenier L, Kuiper I, Van Duijn W, Mieremet-Ooms MA, van Hogezand RA, Lamers CB, Verspaget HW: Imbalanced secondary mucosal antioxidant response in inflammatory bowel disease. J Pathol 201:17–27, 2003

    Google Scholar 

  9. Buffinton GD, Doe WF: Depleted mucosal antioxidant defences in inflammatory bowel disease. Free Rad Biol Med 19:911–918, 1995

    Google Scholar 

  10. Ahnfelt-Ronne I, Nielssen OH: The anti-inflammatory moiety of sulfasalzine, 5-aminosalicylic acid, is a radical scavanger. Agents Actions 21:191–194, 1987

    Google Scholar 

  11. Barbosa DS, Cecchini R, El Kadri MZ, Rodriguez MA, Burini RC, Dichi I: Decreased oxidative stress in patients with ulcerative colitis supplemented with fish oil omega-3 fatty acids. Nutrition 19:837–842, 2003

    Google Scholar 

  12. Hirayama A, Nagase S, Ueda A, Ishizu T, Taru Y, Yoh K, Hirayama K, Kobayashi M, Koyama A: Oxidative stress during leukocyte absorption apheresis. J Clin Apheresis 18:61–66, 2003

    Google Scholar 

  13. Kampa M, Nistikaki A, Tsaoussis V, Maliaraki N, Notas G, Castanas E: A new automated method for the determination of the total antioxidant capacity (TAC) of human plasma, based on the crocin bleaching assay. BMC Clin Pathol 2:3, 2002

    Google Scholar 

  14. Lennard-Jones JE: Classification of inflammatory bowel disease. Scand J Gastroenterol Suppl 24:2–6, 1989

    Google Scholar 

  15. Best WR, Becktel JM, Singleton JW, Kern F Jr: Development of a Crohn's disease activity index. National Cooperative Crohn's Disease Study. Gastroenterology 70:439–444, 1976

    Google Scholar 

  16. Walmsley RS, Ayres RC, Pounder RE, Allan RN: A simple clinical colitis activity index. Gut 43:29–32, 1998

    Google Scholar 

  17. Severin E, Nave B, Stander M, Ott R, Traupe H: Total antioxidative capacity is normal in sera from psoriasis patients despite elevated bilirubin, tocopherol and urate levels. Dermatology198:336–339, 1999

    Google Scholar 

  18. Malliaraki N, Mpliamplias D, Kampa M, Perakis K, Margioris AN, Castanas E: Total and corrected antioxidant capacity in hemodia-lyzed patients. BMC Nephrology 4:4, 2003

    Google Scholar 

  19. Tuzun A, Erdil A, Inal V, Aydin A, Bagci S, Yesilova Z, Sayal A, Karaeren N, Dagalp K: Oxidative stress and antioxidant capacity in patients with inflammatory bowel disease. Clin Biochem 35:569–572, 2002

    Google Scholar 

  20. Mulder TP, Verspaget HW, Janssens AR, DeBruin PAF, Pena AS, Lamers CBHW: Decrease in two intestinal Cu/Zn containing proteins with antioxidant function in inflammatory bowel disease. Gut 32:1146–1150, 1991

    Google Scholar 

  21. Dagli U, Balk M, Yucel D, Ulker A, Over H, Saydam G, Sahin B: The role of reactive oxygen metabolites in ulcerative colitis. Inflamm Bowel Dis 3:260–264, 1997

    Google Scholar 

  22. Lih-Brody L, Powell SR, Collier KP, Reddy GM, Cerchia R, Kahn E, Weissman GS, Katz S, Floyd RA, McKinley MJ, Fisher SE, Mullin GE: Increased oxidative stress and decreased antioxidant defenses in mucosa of inflammatory bowel disease. Dig Dis Sci 41:2078–2086, 1996

    Google Scholar 

  23. Bhaskar L, Ramakrishna BS, Balasubramanian KA: Colonic mucosal antioxidant enzymes and lipid peroxide levels in normal subjects and patients with ulcerative colitis. J Gastroent Hepatol 10:140–143, 1995

    Google Scholar 

  24. Iantomasi T, Marraccini P, Favilla F, Vincenzini MT, Ferretti P, Tonelli F: Glutathione metabolism in Crohn's disease. Biochem Med Metab Biol 53:87–91, 1994

    Google Scholar 

  25. Sturniolo GC, Mestriner C, Lecis PE, D'Odorico A, Venturi C, Irato P, Cecchetto A, Tropea A, Longo G, D'Inca R: Altered plasma and mucosal concentrations of trace elements and antioxidants in active ulcerative colitis. Scand J Gastroenterol 33:644–649, 1998

    Google Scholar 

  26. Buffinton GD, Doe WF: Altered ascorbic acid status in the mucosa from inflammatory bowel disease patients. Free Radic Res 22:131–143, 1995

    Google Scholar 

  27. Koch TR, Yuan LX, Stryker SJ, Ratliff P, Telford GL, Opara EC: Total antioxidant capacity of colon in patients with chronic ulcerative colitis. Dig Dis Sci 45:1814–1819, 2000

    Google Scholar 

  28. Durak I, Yasa MH, Bektas A, Kacmaz M, Cimen MY, Ozturk HS: Mucosal antioxidant defense is not impaired in ulcerative colitis. Hepatogastroenterology 47:1015–1017, 2000

    Google Scholar 

  29. D'Inca R, Cardin R, Benazzato L, Angriman I, Martines D, Sturniolo GC: Oxidative DNA damage in the mucosa of ulcerative colitis increases with disease duration and displasia. Infamm Bowel Dis 10:23–27, 2004

    Google Scholar 

  30. Seril DN, Liao J, Yang GY, Yang CS: Oxidative stress and ulcerative colitis-associated carcinogenesis: Studies in humans and animal models. Carcinogenesis 24:353–362, 2003

    Google Scholar 

  31. Ghiselli A, Serafini M, Natella F, Scaccini C: Total antioxidant capacity as a tool to assess redox status: Critical viewand experimental data. Free Radic Biol Med 29:1106–1114, 2000

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Department of Gastroenterology, University Hospital Heraklion, Crete, Greece

    Ioannis E. Koutroubakis, Philippos D. Dimoulios, Konstantinos Karmiris & Elias A. Kouroumalis

  2. Department of Clinical Chemistry, University Hospital Heraklion, Crete, Greece

    Niki Malliaraki

  3. Department of Experimental Endocrinology, University Hospital Heraklion, Crete, Greece

    Elias Castanas

Authors
  1. Ioannis E. Koutroubakis
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Niki Malliaraki
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Philippos D. Dimoulios
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Konstantinos Karmiris
    View author publications

    You can also search for this author in PubMed Google Scholar

  5. Elias Castanas
    View author publications

    You can also search for this author in PubMed Google Scholar

  6. Elias A. Kouroumalis
    View author publications

    You can also search for this author in PubMed Google Scholar

Rights and permissions

Reprints and permissions

About this article

Cite this article

Koutroubakis, I.E., Malliaraki, N., Dimoulios, P.D. et al. Decreased Total and Corrected Antioxidant Capacity in Patients with Inflammatory Bowel Disease. Dig Dis Sci 49, 1433–1437 (2004). https://doi.org/10.1023/B:DDAS.0000042242.22898.d9

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:DDAS.0000042242.22898.d9

Search

Navigation