Abstract
Many grape viruses, such as filamentous Grapevine leafroll-associated viruses in the Closteroviridae family, are spread primarily through infected propagating material. However, there is increasing evidence that leafroll disease are spread in the field by insect vectors, namely mealybugs and other scale insects. This study was carried out in the northern wine-growing regions of France where Grapevine leafroll-associated virus-1 and -3 (GLRaV-1 and -3) are the most widespread grape Ampelovirus species. The vineyards were inspected for presence of mealybug and scale insects and grapes infected by GLRaV-1 and -3. Mealybugs, Heliococcus bohemicus, Phenacoccus aceris (Pseudococcidae) and the soft scale insect Parthenolecanium corni (Coccidae), were capable of a transmission efficiency of 14%, 23% and 29% respectively. GLRaV-1 and -3 infections that resulted from virus transmission were confirmed with DAS-ELISA using polyclonal antibodies. This is the first report of GLRaV-1 and -3 transmission by mealybug and coccid species in France, and the first report of the ability of H. bohemicus and Phenacoccus aceris to transmit these viruses to grapevines. The relevance of these findings with regards to maintenance of virus-free grapevine stocks and to control leafroll spread in commercial vineyards is discussed.
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References
Belli G, Fortusini A, Casati P, Belli L, Bianco P and Prati S (1994) Transmission of a grapevine leafroll associated closterovirus by the scale insect Pulvinaria vitis L. Rivista di PatologiaVegetale 4: 105-108
Cabaleiro C and Segura A (1997) Field transmission of grapevine leafroll associated virus 3 (GLRaV-3) by the mealybug Planococcus citri Risso. Plant Disease 81: 283-287
Camporese P (1994) Prime osservazioni sulla biologia di Heliococcus bohemicus Sulc nei vigneti del Veneto. Memorie della Società entomologica Italiana 72: 195-200
Engelbrecht D and Kasdorf G (1990) Transmission of grapevine leafroll disease and assocaited closteroviruses by the vine mealybug, Planococcus ficus. Phytophylactica 22: 341-346
Garau R, Prota VA, Boscia D, Fiori M and Prota U (1995) Pseudococcus affinis Mask., new vector of grapevine trichoviruses A and B. Vitis 34: 67-68
Geiger CA, Daane K, Bentley W, Yokota G and Martin L (2001) Sampling program for grape mealybugs improves pest management. California Agriculture 55: 19-27
Golino DA, Sim ST, Gill R and Rowhani A (2002) California mealybugs can spread grapevine leafroll disease. California Agriculture 56: 196-201
Gullan P (1997) Adaptations in scale insects. Annual Review of Entomology 42: 23-50
Jakab J and Szendrey L (1989) A viaszos akac-pajzstetu (Heliococcus bohemicus Sulc) megjelenese Heves megye szoloultetvényeiben (On the presence of Heliococcus bohemicus Sulc in vineyards of the Héves region) (in Hungarian). Növényvédelem XXV Evflolyam: 460-464
Kosztarab M and Kozar F (1988) Scale insects of central Europe. In: Spencer KA (ed) Series Entomologica, Vol 41 Kluwer Academic Publishers, Dordrecht, the Netherlands
Martelli GP (1986) Virus and virus-like disease of the grapevine in the Mediterranean area. FAO Plant Protection Bulletin 34: 25-42
Martelli G, Agranovsky AA, Bar-Joseph M, Boscia D, Candresse T, Coutts RHA, Dolja V, Falk B, Gonsalves D, Jelkmann W, Karasev A, Minafra A, Namba S, Vetten H, Wisler G and Yoshikawa N (2002) The family Closteroviridae revised. Archives of Virology 147: 2039-2044
Pellizzari-Scaltritti G (1997) Coccid pests of important crops: Grapevine. In: Ben-Dov Y and Hodgson C (eds) Soft Scale Insects-Their Biology, Natural Enemies and Control. (pp 323-331) Elsevier Science, Amsterdam & New York
Petersen C and Charles J (1997) Transmission of grapevine leafroll-associated closteroviruses by Pseudococcus longispinus and P. calceolariae. Plant Pathology 46: 509-515
Raine J, McMullen RD and Farbes AR (1986) Transmission of the agent causing little cherry disease by the apple mealybug Phenacoccus aceris and the dodder Cuscuta lupuliformis. Canadian Journal of Plant Pathology 8: 6-11
Rohrbach K, Beardsley J, German T, Reimer N and Sanford W (1988) Mealybug wilt, mealybugs and ants on pineapple. Plant Disease 72: 558-565
Rosciglione B and Castellano M (1985) Further evidence that mealybugs can transmit grapevine virusA(GVA) to herbaceous hosts. Phytopathologia Mediterranea 24: 186-188
Rosciglione B and Gugerli P (1989) Transmission of grapevine leafroll disease and an associated closterovirus to healthy grapevine by the mealybug Planococcus ficus. Phytoparasitica 17: 63
Sforza R (2000) Les cochenilles sur la vigne: bio-éthologie, Impact agronomique, lutte et prophylaxie. In: Stockel J (ed) Les Ravageurs de la Vigne (pp 130-147) Feret, Bordeaux, France
Sforza R, Delvare G, Sentenac G, Kuntzmann P and Lanthiome D (2003) Inventaire et évaluation des antagonistes de cochenilles sur la vigne. Phytoma-La défense des végétaux 558: 42-46
Tanne E, Ben-Dov Y and Raccah B (1989) Transmission of closterolike particles associated with grapevine leafroll by mealybugs (Pseudococcidae) in Israel. Phytoparasitica 17: 64
Zimmermann D, Bass P, Legin R and Walter B (1990) Characterization and serological detection of four closterovirus-like particles associated with leafroll disease on grapevine. Journal of Phytopathology 130: 205-218
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Sforza, R., Boudon-Padieu, E. & Greif, C. New Mealybug Species Vectoring Grapevine Leafroll-Associated Viruses-1 and -3 (GLRaV-1 and -3). European Journal of Plant Pathology 109, 975–981 (2003). https://doi.org/10.1023/B:EJPP.0000003750.34458.71
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DOI: https://doi.org/10.1023/B:EJPP.0000003750.34458.71