Skip to main content
Log in

FUSARIUM-ID v. 1.0: A DNA Sequence Database for Identifying Fusarium

  • Published:
European Journal of Plant Pathology Aims and scope Submit manuscript

Abstract

One of the greatest impediments to the study of Fusarium has been the incorrect and confused application of species names to toxigenic and pathogenic isolates, owing in large part to intrinsic limitations of morphological species recognition and its application. To address this problem, we have created FUSARIUM-ID v. 1.0, a publicly available database of partial translation elongation factor 1-alpha (TEF) DNA sequences, presently representing a selected sample of the diversity of the genus diversity, with excellent representation of Type-B trichothecene toxin producers, and the Gibberella fujikuroi, Fusarium oxysporum and F. solani species complexes. Users can generate sequences using primers that are conserved across the genus, and use the sequence as a query to BLAST the database, which can be accessed at http://fusarium.cbio.psu.edu, or in a phylogenetic analysis. Correct identification of a known species in these groups often can be performed using this gene region alone. This growing database will contain only vouchered sequences attached to publicly available cultures. In the future, FUSARIUM-ID will be expanded to include additional sequences, including multiple sequences from the same species, sequences from new and revised species, and information from additional genes.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Aoki T and O'Donnell KL (1998) Fusarium kyushuense sp. nov. from Japan. Mycoscience 39: 1-6

    Google Scholar 

  • Aoki T and O'Donnell K (1999) Morphological and molecular characterization of Fusarium pseudograminearum sp nov., formerly recognized as the Group 1 population of F. graminearum. Mycologia 91: 597-609

    Google Scholar 

  • Aoki T, O'Donnell K, Homma Y and Lattanzi AR (2003) Sudden-death syndrome of soybean is caused by two morphologically and phylogenetically distinct species within the Fusarium solani species complex-F. virguliforme in North America and F. tucumaniae in South America. Mycologia 95: 660-684

    Google Scholar 

  • Cho SW, Mitchell A, Regier JC, Mitter C, Poole RW, Friedlander TP and Zhao SW (1995) A highly conserved nuclear gene for low-level phylogenetics-Elongation Factor-1-Alpha recovers morphology-based tree for Heliothine moths. Molecular Biology and Evolution 12: 650-656

    CAS  PubMed  Google Scholar 

  • Fessehaie A, De Boer SH and Levesque CA (2003) An oligonucleotide array for the identification and differentiation of bacteria pathogenic on potato. Phytopathology 93: 262-269

    CAS  Google Scholar 

  • Geiser DM (2003) Practical molecular taxonomy of fungi. In: Lange L and Tkacz J (eds) Advances in Fungal Biotechnology for Industry, Medicine and Agriculture, Kluwer Academic Publishers, Dordrecht, The Netherlands

    Google Scholar 

  • Geiser DM, Juba JH, Wang B and Jeffers SN (2001) Fusarium hostae sp nov., a relative of F. redolens with a Gibberella teleomorph. Mycologia 93: 670-678

    Google Scholar 

  • Marasas et al. (1984)

  • Mitchell A, Cho S, Regier JC, Mitter C, Poole RW and Matthews M (1997) Phylogenetic utility of elongation factor-1 alpha in Noctuoidea (Insecta: Lepidoptera): The limits of synonymous substitution. Molecular Biology and Evolution 14: 381-390

    CAS  PubMed  Google Scholar 

  • O'Donnell K (2000) Molecular phylogeny of the Nectria haematococca-Fusarium solani species complex. Mycologia 92: 919-938

    Google Scholar 

  • O'Donnell K and Cigelnik E (1997a) Two divergent intragenomic rDNA ITS2 types within a monophyletic lineage of the fungus Fusarium are nonorthologous. Molecular and Phylogenetic Evolution 7: 103-116

    Google Scholar 

  • O'Donnell K and Cigelnik E (1997b) Phylogenetic relationships among ascomycetous truffles and the true and false morels from 18S and 28S ribosomal DNA sequence analysis. Mycologia 89: 48-65

    Google Scholar 

  • O'Donnell K, Cigelnik E and Nirenberg HI (1998a) Molecular systematics and phylogeography of the Gibberella fujikuroi species complex. Mycologia 90: 465-493

    Google Scholar 

  • O'Donnell K, Cigelnik E and Casper HH (1998b) Molecular phylogenetic, morphological, and mycotoxin data support reidentification of the Quorn mycoprotein fungus as Fusarium venenatum. Fungal Genetics and Biology 23: 57-67

    Article  PubMed  Google Scholar 

  • O'Donnell K, Kistler HC, Cigelnik E and Ploetz RC (1998c) Multiple evolutionary origins of the fungus causing Panama disease of banana: Concordant evidence from nuclear and mitochondrial gene genealogies. Proceedings of the National Academy of Sciences of the United States of America 95: 2044-2049

    Article  PubMed  Google Scholar 

  • O'Donnell K, Nirenberg HI, Aoki T and Cigelnik E (2000a) A multigene phylogeny of the Gibberella fujikuroi species complex: Detection of additional phylogenetically distinct species. Mycoscience 41: 61-78

    Google Scholar 

  • O'Donnell K, Kistler HC, Tacke BK and Casper HH (2000b) Gene genealogies reveal global phylogeographic structure and reproductive isolation among lineages of Fusarium graminearum, the fungus causing wheat scab. Proceedings of the National Academy of Sciences of the United States of America 97: 7905-7910

    Article  PubMed  Google Scholar 

  • Snyder WC and Hansen HN (1940) The species concept in Fusarium. American Journal of Botany 27: 64-67

    Google Scholar 

  • Snyder WC and Hansen HN (1941) The species concept in Fusarium with reference to section Martiella. American Journal of Botany 28: 738-742

    Google Scholar 

  • Snyder WC and Hansen HN (1945) The species concept in Fusarium with reference to Discolor and other sections. American Journal of Botany 32: 657-666

    Google Scholar 

  • Summerell BA, Salleh B and Leslie JF (2003) A utilitarian approach to Fusarium identification. Plant Disease 87: 117-128

    Google Scholar 

  • Taylor JW, Jacobson DJ, Kroken S, Kasuga T, Geiser DM, Hibbett DS and Fisher MC (2000) Phylogenetic species recognition and species concepts in fungi. Fungal Genetics and Biology 31: 21-32

    Article  CAS  PubMed  Google Scholar 

  • Ward TJ, Bielawski JP, Kistler HC, Sullivan E and O'Donnell K (2002) Ancestral polymorphism and adaptive evolution in the trichothecene mycotoxin gene cluster of phytopathogenic Fusarium. Proceedings of the National Academy of Sciences of the United States of America 99: 9278-9283

    CAS  PubMed  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Rights and permissions

Reprints and permissions

About this article

Cite this article

Geiser, D.M., del Mar Jiménez-Gasco, M., Kang, S. et al. FUSARIUM-ID v. 1.0: A DNA Sequence Database for Identifying Fusarium . European Journal of Plant Pathology 110, 473–479 (2004). https://doi.org/10.1023/B:EJPP.0000032386.75915.a0

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:EJPP.0000032386.75915.a0

Navigation