Abstract
Leaf developmental behavior in rice (Oryza sativa L.) is one of the important agronomic characteristics, which not only determines vegetative growth but also influences grain yield. This study was conducted to identify quantitative trait loci (QTLs) for total number of leaves (TNL), days to the emergence of flag-leaf (DEF) and the leaf emergence rates (LER) on main stem, which mainly represent leaf developmental behavior, using recombinant inbred lines (RILs) derived from a cross between a japonica variety, Asominori and an indica variety, IR24, cultivated in 2001 and 2002. The transgressive segregations in both parental directions and continuous variations of all three tested traits were observed. Significant correlations among these traits were detected. A total of fourteen QTLs for leaf development behavior were detected with 289 RFLP markers. Six QTLs controlling TNL were mapped to chromosomes 3, 5, 6, 8, 9, 12, and accounted for 5.6∼15.7% of the total phenotypic variations, and three QTLs for DEF were mapped to chromosome 3, 6, 8 and accounted for 10.7∼35.4% of total phenotype variation and five QTLs for LER were mapped to chromosome 1(two QTLs), 2, 4, 9 and explained 6.2∼17.5% of phenotype variation. The identification of QTLs for leaf developmental behavior in rice may be useful for selection of ‘fast growing genotype’ before heading using maker-assisted selection.
Similar content being viewed by others
References
Dong, Y., E. Tsuzuki, H. Kamiunten, H. Terao, D. Lin, M. Matsuo & Y. Zheng, 2003a. Identification of quantitative trait loci associated with pre-harvest sprouting resistance in rice (Oryza sativa L.) Field Crops Res 81:133–139.
Dong, Y., E. Tsuzuki, H. Kamiunten, H. Terao & D. Lin, 2003b. Mapping of QTL for embryo size in rice. Crop Sci 43: 1068–1071.
Dong, Y., Y. Zheng, E. Tsuzuki & H. Terao, 2002. Quantitative trait loci controlling steamed-rice shape in a recombinant inbred pop-ulation. Int Rice Res Note 27: 19–20.
Kubo, T. & A. Yoshimura, 2002. Genetic basis of hybrid breakdown in a Japonica/Indica cross of rice, Oryza sativa L. Theor Appl Genet 105:906–911.
Li, Z.K., A. H. Paterson & S.R.M. Pinson 1999. RFLP facilitated analysis of tiller and leaf angles in rice (Oryza sativa L.) Euphytica 109(2): 79–84.
McCouch, S.R., Y.G. Cho, M. Yano, E. Paul & M. Blinstrub, 1997. Report on QTL nomenclature. Rice Genetics Newsletter 14:11–13.
Sasahara, H., Y. Fukuta & T. Fukuyama, 1999. Mapping of QTLs for vascular bundle system and spike morphology in rice, Oryza sativa L. Breed Sci 49:75–81.
Tanksley, S.D., 1993. Mapping polygenes. Annu Rev Genet 27:205–233.
Teng, S., Q. Qian, D. Zeng, Y. Kunihiro, K. Fujimoto, D. Huang & L.Zhu, 2004. QTL analysis of leaf photosynthetic rate and re-lated physiological traits in rice (Oryza sativa L.). Euphytica 135: 1–7
Tsunematsu, H., A. Yoshimura, Y. Harushima, Y. Nagamura, N. Kurata, M. Yano & N. Iwata, 1996. RFLP framework map us-ing recombinant inbred lines in rice. Breeding Science 46:279–284.
Wang S., C. J. Basten & Z. Zeng, 2003. Windows QTL Cartographer 2.0. Department of Statistics, North Carolina State University, Raleigh, NC. (http://statgen.ncsu.edu/qtlcart/WQTLCart.htm)
Yamagata, H., 1997. Differentiation and inheritance of characters. Chapter 2. Inheritance of morphological characters. In Science of the Rice Plant, Genetics (Vol.3) Matsuo, T., Y. Futsuhara, F. Kikuchi and H. Yamaguchi (eds), Food and Agriculture Policy Research Center, Tokyo, Japan, 277–285.
Yamazaki, M., H. Tsunematstu, A. Yoshimura, N. Iwata & H. Yasui, 1999. Quantitative trait locus mapping of ovicidal response in rice (Oryza sativa L.) against whitebacked planthopper (Sogatella furcifera Horvath). Crop Sci 39:1178–1183.
Yamazaki, M., A. Yoshimura & H. Yasui, 2000. Mapping of quantitative trait loci of ovicidal response to brown planthop-per( Nilaparvata lugen Stal) in rice (Oryza sativa L.). Breed Sci 50:291–296.
Yan J.Q, J. Zhu, C. He, M. Benmoussa & P. Wu, 1998. Quanti-tative trait loci analysis for the development behavior of num-ber tiller in rice (Oryza sativa L.). Theor Appl Genet 97:267–274.
Yan J.Q, J. Zhu, C. He, M. Benmoussa & P. Wu, 1999. Molecular Marker-assisted dissection of genotype xenvironment interaction for plant type traits in rice (Oryza sativa L.) Crop Sci 39: 538–544.
Yano, M. & T. Sasaki, 1997. Genetic and molecular dissection of quantitative traits in rice. Plant Mol Biol 35:145–153.
Yoshimura, A, M. Okamoto, T. Nagamine & H. Tsunematsu, 1998. Rice QTL analysis for days to heading-under the cultivation of Ishigaki island. Breed Sci 48 (suppl 1.): 73.
Zheng, Y., Y. Dong, A. Matsui, T. Udatsu & H. Fujiwara, 2003. Molecular genetic basis of determining subspecies of ancient rice using the shape of phytoliths. J Archaeological Sci 30: 1215-1221.
Author information
Authors and Affiliations
Rights and permissions
About this article
Cite this article
Dong, Y., Kamiunten, H., Ogawa, T. et al. Mapping of QTLs for leaf developmental behavior in rice (Oryza sativa L.). Euphytica 138, 169–175 (2004). https://doi.org/10.1023/B:EUPH.0000046799.21410.13
Issue Date:
DOI: https://doi.org/10.1023/B:EUPH.0000046799.21410.13