Abstract
The spectral properties of a rigidified trimethine cyanine dye, Cy3B™ have been characterised. This probe has excellent fluorescent properties, good water solubility and can be bioconjugated. The emission properties of this fluorophore have also been investigated upon conjugation to an antibody. This study compared the conjugated emission properties of Cy3B with other commercially available fluorophores emitting at similar wavelengths.
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Cooper, M., Ebner, A., Briggs, M. et al. Cy3B™: Improving the Performance of Cyanine Dyes. Journal of Fluorescence 14, 145–150 (2004). https://doi.org/10.1023/B:JOFL.0000016286.62641.59
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DOI: https://doi.org/10.1023/B:JOFL.0000016286.62641.59