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Mining Microarrays for Metabolic Meaning: Nutritional Regulation of Hypothalamic Gene Expression

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Abstract

DNA microarray analysis has been used to investigate relative changes in the level of gene expression in the CNS, including changes that are associated with disease, injury, psychiatric disorders, drug exposure or withdrawal, and memory formation. We have used oligonucleotide microarrays to identify hypothalamic genes that respond to nutritional manipulation. In addition to commonly used microarray analysis based on criteria such as fold-regulation, we have also found that simply carrying out multiple t tests then sorting by P value constitutes a highly reliable method to detect true regulation, as assessed by real-time polymerase chain reaction (PCR), even for relatively low abundance genes or relatively low magnitude of regulation. Such analyses directly suggested novel mechanisms that mediate effects of nutritional state on neuroendocrine function and are being used to identify regulated gene products that may elucidate the metabolic pathology of obese ob/ob, lean Vgf-/Vgf-, and other models with profound metabolic impairments.

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Authors and Affiliations

  1. Fishberg Research Center for Neurobiology, Mount Sinai School of Medicine, New York, New York

    Kelvin Yen, Jason Mastaitis, Ha Nguyen, Elizabeth Watson, Stuart C. Sealfon & Stephen R. J. Salton

  2. Kastor Neurobiology of Aging Laboratories, Mount Sinai School of Medicine, New York, New York

    Charles V. Mobbs, Kelvin Yen, Jason Mastaitis & Ha Nguyen

  3. Department of Geriatrics, Mount Sinai School of Medicne, New York, New York

    Charles V. Mobbs & Stephen R. J. Salton

  4. Department of Neurology, Mount Sinai School of Medicine, New York, New York

    Elisa Wurmbach & Stuart C. Sealfon

  5. Graduate Training Program in Neuroscience, Mount Sinai School of Medicine, New York, New York

    Kelvin Yen, Jason Mastaitis, Ha Nguyen & Elizabeth Watson

  6. Department of Environmental Medicine, Center for Functional Genomics, University of Rochester Medical Center, Rochester, New York

    Andrew Brooks

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Mobbs, C.V., Yen, K., Mastaitis, J. et al. Mining Microarrays for Metabolic Meaning: Nutritional Regulation of Hypothalamic Gene Expression. Neurochem Res 29, 1093–1103 (2004). https://doi.org/10.1023/B:NERE.0000023596.49140.e0

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