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Hyperhydricity of Micropropagated Shoots: A Typically Stress-induced Change of Physiological State

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Abstract

Hyperhydricity of micropropagated shoots, formerly called vitrification, undoubtedly results from growth and culture conditions, subjectively reputated as stressing factors: wounding, infiltration of soft culture medium, generally of a high ionic strength, rich in nitrogen and in growth regulators in a special balance, in a humid and gaseous confined atmosphere. Stress is (objectively) defined as a disruption of homeostasis resulting from a constraint escaping the usual flexibility of metabolism. It induces another temporary (reversible) or definitive (irreversible) thermodynamic physiological state. The state-change concept developed by Strasser (1988) and Strasser and Tsimilli-Michael (2001) is applicable to the phenomenon of hyperhydricity. An appraisal of the redox capacities of hyperhydrated shoots together with a study of some enzymic activities that catalyse pentose phosphate and glycolytic pathways has indeed shown that such shoots have evolved towards a temporary state of lower differentiation or a juvenile state with a sufficient activity to survive and to defend themselves.

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Authors and Affiliations

  1. Laboratoire de Biologie moléculaire et d'Hormonologie végétales, Université de Liège, Institut de Botanique B 22, Sart Tilman, BE-4000, Liège, Belgium

    Claire Kevers, Thierry Franck, Jacques Dommes & Thomas Gaspar

  2. Laboratoire de Bioénergétique, Université de Genève, Chemin des Embrouchis, 10, CH-1254, Jussy-Geneva, Switzerland

    Reto J. Strasser

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  1. Claire Kevers
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  2. Thierry Franck
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  3. Reto J. Strasser
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  4. Jacques Dommes
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  5. Thomas Gaspar
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Correspondence to Claire Kevers.

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Kevers, C., Franck, T., Strasser, R.J. et al. Hyperhydricity of Micropropagated Shoots: A Typically Stress-induced Change of Physiological State. Plant Cell, Tissue and Organ Culture 77, 181–191 (2004). https://doi.org/10.1023/B:TICU.0000016825.18930.e4

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