Skip to main content
SpringerLink
Log in

In vitro Shoot Regeneration from Leaves of Sweet Cherry (Prunus avium) 'Lapins' and 'Sweetheart'

  • Published:
Plant Cell, Tissue and Organ Culture Aims and scope Submit manuscript

Abstract

Shoot regeneration was achieved from leaves of in vitro cultures of Prunus avium L. cv. 'Lapins' and 'Sweetheart' using woody plant medium (WPM) supplemented with 1-naphthalene-acetic acid (NAA) and thidiazuron (TDZ) or benzyladenine (BA). Percent regeneration was influenced by plant growth regulators and by explant type, orientation and wounding. Optimal regeneration was observed with whole-leaf explants wounded by transverse cuts along the midrib and incubated abaxial surfaces uppermost, on media supplemented with 2.27 or 4.54 µM TDZ plus 0.27 µM NAA. The percent regeneration of the two cultivars was not significantly different. Optimum conditions for regeneration resulted in 71.4% of 'Lapins' and 54% of 'Sweetheart' explants producing one or more shoots per explant.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

Similar content being viewed by others

References

  • Bassi G & Cossio F (1991) In vitro shoot regeneration of 'Blufre' and 'susina di Dro' prune cultivars (Prunus domestica L.). Acta Hort. 289: 82–82

    Google Scholar 

  • Bassi G & Cossio F (1994) Simplified protocol for in vitro shoot regeneration from leaves of Prunus domestica L. (cv. 'susina di Dro'). In: Schmidt H & Kellerhals M (eds) Progress in Temperate Fruit Breeding (pp. 361–363). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Caboni E, Tonelli MG, Lauri P, Angeli SD & Damiano C (1999) In vitro regeneration from leaves of wild pear. Plant Cell Tiss. Org. Cult. 59: 1–7

    Google Scholar 

  • Chevreau E & Skirvin RM (1992) Pear. In: Janick J & Moore JN (eds) Fruit Breeding Vol. 3, Tree and Tropical Fruits (pp. 263–276). Wiley, New York

    Google Scholar 

  • Driver JA & Kuniyuki AH (1984) In vitro propagation of Paradox walnut rootstock. HortScience 19: 507–509

    Google Scholar 

  • Escalettes V & Dosba F (1993) In vitro adventitious shoot regeneration from leaves of Prunus spp. Plant Sci. 90: 201–209

    Google Scholar 

  • Fasolo F, Zimmerman RH & Fordham I (1989) Adventitious shoot formation on excised leaves of in vitro grown shoots of apple cultivars. Plant Cell Tiss. Org. Cult. 16: 75–87

    Google Scholar 

  • Feucht W & Dausend B (1976) Root induction in vitro of easy-toroot Prunus pseudocerasus and difficult-to-root Prunus avium. Scientia Hort. 4: 49–54

    Google Scholar 

  • Grant NJ & Hammatt N (2002) Adventitious shoot development from wild cherry (Prunus avium L.) leaves. New Forests 20: 287–295

    Google Scholar 

  • Hammatt N (1993) Micropropagation of fastigate bird cherry (Prunus padus L.) and adventitious shoot formation from leaves. J. Hort. Sci. 68: 975–982

    Google Scholar 

  • Hammatt N & Grant NJ (1998) Shoot regeneration from leaves of Prunus serotina Ehrh. (black cherry) and P. avium L. (wild cherry). Plant Cell Rep. 17: 526–530

    Google Scholar 

  • Hammerschlag FA, Bauchan G & Scorza R (1985) Regeneration of peach plants from callus derived from immature embryos. Theor. Appl. Genet. 70: 248–251

    Google Scholar 

  • Hinchee MAW, Corbin DR, Armstrong CL, Fry JE, Sato SS, DeBoer DL, Peterson WL, Armstrong TA, Connor-Ward DV, Layton JG & Horsch RB (1994) Plant transformation. In: Vasil IK & Thorpe TA (eds) Plant Cell and Tissue Culture (pp. 231–270). Kluwer Academic Publishers, Dordrecht

    Google Scholar 

  • Huetteman CA & Preece JE (1993) Thidiazuron: a potent cytokinin for woody plant tissue culture. Plant Cell Tiss. Org. Cult. 33: 105–119

    Google Scholar 

  • Lane WD & Cossio F (1986) Adventitious shoots from cotyledons of immature cherry and apricot embryos. Can. J. Plant Sci. 66: 953–959

    Google Scholar 

  • Lloyd G & McCown LB (1980) Commercially-feasible micropropagation of mountain laurel, Kalmia latifolia, by use of shoot-tip culture. Comb. Proc. Int. Plant Prop. Soc. 30: 421–427

    Google Scholar 

  • Mante SR, Scorza R & Cordis JM (1989) Plant regeneration from cotyledons of Prunus persica, Prunus domestica and Prunus cerasus. Plant Cell Tiss. Org. Cult. 19: 1–11

    Google Scholar 

  • Mante S, Morgens PH, Scorza R, Cordts JM & Callahan AM (1991) Agrobacterium-mediated transformation of plum (Prunus domestica L.) hypocotyl slices and regeneration of transgenic plants. Bio/Technology 9: 853–857

    Google Scholar 

  • Mehra A & Mehra PN (1974) Organogenesis and plantlet formation in vitro in almond. Bot. Gaz. 135: 61–73

    Google Scholar 

  • Miguel CM & Oliveira MM (1999) Transgenic almond (Prunus dulcis Mill.) plants obtained by Agrobacterium-mediated transformation of leaf explants. Plant Cell Reports 18: 387–393

    Google Scholar 

  • Miguel CM, Druart P & Oliveira MM (1996) Shoot regeneration from adventitious buds induced on juvenile and adult almond (Prunus dulcis Mill.) explants. In Vitro Cell Dev. Biol.-Plant 32: 148–153

    Google Scholar 

  • Mok MC, Mok DWS, Turner JE & Muser CV (1987) Biological and biochemical effects of cytokinin-active phenylurea derivatives in tissue culture systems. HortScience 22: 1194–1197

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant. 15: 473–497

    Google Scholar 

  • Pieterse RE (1989) Regeneration of plants from callus and embryo of 'Royal' Apricot. Plant Cell Tiss. Org. Cult. 19: 175–179

    Google Scholar 

  • Pooler MR & Scorza R (1995) Regeneration of peach (Prunus persica L. Batsch) rootstock cultivars from cotyledons of mature stored seeds. HortScience 30: 355–356

    Google Scholar 

  • Quoirin M & Lepoivre P (1977) Improved media for in vitro culture of Prunus sp. Acta Horti. 78: 437–442

    Google Scholar 

  • SAS Institute (1989) SAS/STAT User's Guide. 4th edn. ver. 6. SAS Institute Inc., Cary, North Carolina

    Google Scholar 

  • Scorza R (1991) Gene transfer for the genetic improvement of perennial fruit and nut crops. HortScience 26: 1033–1035

    Google Scholar 

  • Snir I (1982) In vitro propagation of sweet cherry cultivars. Hort-Science 17: 192–193

    Google Scholar 

  • Tang H, Ren Z, Reustle G & Krczal G (2000) Plant regeneration from leaves of sweet and sour cherry cultivars. Scientia Hort. 93: 235–244

    Google Scholar 

  • Welander M (1988) Plant regeneration from leaf and stem segments of shoots raised in vitro from mature apple trees. J. Plant Physiol. 132: 738–744

    Google Scholar 

  • Yang HY & Schmidt H (1992) Untersuchungen zur Adventivsproßregeneration in vitro bei Kirschen. II. Adventivsproßbildung an in vitro-Blättern verschiedener Prunus avium-Idio-typen. Gartenbauwissenschaft 57: 7–10

    Google Scholar 

Download references

Author information

Authors and Affiliations

  1. Pacific Agri-Food Research Centre, Agriculture and Agri-Food Canada, Summerland, BC, V0H 1Z0, Canada

    B. Bhagwat & W. David Lane

Authors
  1. B. Bhagwat
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. W. David Lane
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to B. Bhagwat.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Bhagwat, B., David Lane, W. In vitro Shoot Regeneration from Leaves of Sweet Cherry (Prunus avium) 'Lapins' and 'Sweetheart'. Plant Cell, Tissue and Organ Culture 78, 173–181 (2004). https://doi.org/10.1023/B:TICU.0000022552.12449.71

Download citation

  • Issue Date:

  • DOI: https://doi.org/10.1023/B:TICU.0000022552.12449.71

Search

Navigation