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Effect of Genotype and Explant Age on Callus Induction and Subsequent Plant Regeneration from Root-derived Callus of Indica Rice Genotypes

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Abstract

An attempt has been taken to establish an efficient plant regeneration system in vitro from 3, 5, 7 and 9-days-old root segments of four Indica (Bangladeshi) rice genotypes. Genotypic effects were observed in callus induction and subsequent plant regeneration. Moreover, the stage of development of the root explants also played a significant role in callus formation and subsequent plant regeneration. Younger explants were more efficient in both callus induction and plant regeneration. Plants regenerated in vitro were successfully established in soil and produced fertile seeds.

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References

  • Abe T & Futsuhara Y (1984) Varietal difference of plant regeneration from root callus tissues in rice. Jap. J. Breed. 34: 147–155

    Google Scholar 

  • Abe T & Futsuhara Y (1985) Efficient plant regeneration by somatic embryogenesis from root callus tissues of rice (Oryza sativa L.). J. Plant Physiol. 121: 111–118

    CAS  Google Scholar 

  • Abe T & Futsuhara Y (1986) Genotypic variability for callus formation and plant regeneration in rice (Oryza sativa L.). Theor. Appl. Genet. 72: 3–10

    Article  Google Scholar 

  • Adkins SW (1992) Cereal tissue cultures: control of headspace gases can optimize the conditions for callus proliferation. Aust. J. Bot. 40: 737–749

    Article  CAS  Google Scholar 

  • Basu S, Gangopadhyay G, Mukherjee BB & Gupta S (1997) Plant regeneration of salt adapted callus of Indica rice (var. Basmati 370) in saline conditions. Plant Cell Tiss. Org. Cult. 50: 153–159

    Article  CAS  Google Scholar 

  • Chand S & Sahrawat AK (2000) Efficient plant regeneration from root callus tissues of barley (Hordeum vulgare L.). J. Plant Physiol. 156: 401–407

    CAS  Google Scholar 

  • Chowdhry CN, Tyagi AK, Maheshwari N & Maheshwari SC (1993) Effect of L-proline and L-tryptophan on somatic embryogenesis and plantlet regeneration of rice (Oryza sativa L. cv. Pusa 169). Plant Cell Tiss. Org. Cult. 32: 357–361

    Article  CAS  Google Scholar 

  • Chu QR & Croughan TP (1990) Genetics of plant regeneration in immature panicle culture of rice. Crop Sci. 30: 1194–1197

    Google Scholar 

  • Hartke S & Lörz H (1989) Somatic embryogenesis and plant regeneration from various Indica rice (Oryza sativa L.) genotypes. J. Genet. Breed. 43: 205–214

    Google Scholar 

  • Jain RK (1997) Effect of some factors on plant regeneration from Indica rice cells and protoplasts - a review. Ind. J. Exp. Biol. 35: 323–331

    CAS  Google Scholar 

  • Kawata SI & Ishihara A (1968) The regeneration of rice plant, Oryza sativa L., in the callus derived from the seminal root. Proc. Jap. Acad. 44: 549–553

    Google Scholar 

  • Khanna HK & Raina SK (1998) Genotype × culture media interaction effects on regeneration response of three Indica rice cultivars. Plant Cell Tiss. Org. Cult. 52: 145–153

    Article  CAS  Google Scholar 

  • Kunanuvatchaidach R, Godwin ID & Adkins SW (1995) High effi-ciency plant regeneration from callus induced on mature Indica rice caryopses. Aust. J. Bot. 43: 337–348

    Article  Google Scholar 

  • Lee SH, Shon YG, Lee SI, Kim CY, Koo JC, Lim CO, Choi YJ, Han CD, Chung CH, Choe ZR & Cho MJ (1999) Cultivar variability in the Agrobacterium-rice cell interaction and plant regeneration. Physiol. Plant 107: 338–345

    Article  CAS  Google Scholar 

  • Linsmaier EM & Skoog F (1965) Organic growth factor requirements of tobacco tissue culture. Physiol. Plant 18: 100–127

    CAS  Google Scholar 

  • Morrish F, Vasil V & Vasil IK (1987) Developmental morphogenesis and genetic manipulation in tissue and cell cultures of the gramineae. Adv. Genet. 24: 432–499

    Google Scholar 

  • Murashige T & Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol. Plant 15: 473–497

    CAS  Google Scholar 

  • Peng J & Hodges TK (1989) Genetic analysis of plant regeneration in rice (Oryza sativa L.). In Vitro Cell Dev. Biol. 25: 91–94

    Google Scholar 

  • Rueb S, Leneman M, Schilperoot RA & Hensgens LAM (1994) Efficient plant regeneration through somatic embryogenesis from callus induced on mature rice embryos (Oryza sativa L.). Plant Cell Tiss. Org. Cult. 36: 259–264

    Article  Google Scholar 

  • Vasil IK & Vasil V (1986) Regeneration in cereals and other grass species. In: Vasil IK (ed) Cell Culture and Somatic Cell Genetics of Plants, Vol. 3 (pp. 121–150). Academic Press, New York

    Google Scholar 

  • Wang CC, Sun CS, Chu CC & Wu SC (1978) Studied on the albino pollen plantlets of rice. In: Hu H (ed) Proc. Symp. Plant Tissue Culture (pp. 149–160). Science Press, Beijing

    Google Scholar 

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Authors and Affiliations

  1. Biotechnology Division, Bangladesh Rice Research Institute, Gazipur, 1701, Bangladesh

    Md. Enamul Hoque

  2. Department of Agriculture Sciences, Imperial College London, Wye campus, Ashford, Kent, TN25 5AH, UK

    John W. Mansfield

Authors
  1. Md. Enamul Hoque
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  2. John W. Mansfield
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Correspondence to John W. Mansfield.

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Hoque, M.E., Mansfield, J.W. Effect of Genotype and Explant Age on Callus Induction and Subsequent Plant Regeneration from Root-derived Callus of Indica Rice Genotypes. Plant Cell, Tissue and Organ Culture 78, 217–223 (2004). https://doi.org/10.1023/B:TICU.0000025640.75168.2d

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  • DOI: https://doi.org/10.1023/B:TICU.0000025640.75168.2d

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