Abstract
Leaf segments excised from Centella asiatica, a medicinal and neutraceutical plant, produced abundant somaticembryoswhen cultured onMS mediumwith 9.29 μMkinetin in combination with 2.26 μM2,4-D. Granular, white,shiny clusters of callus developed after 1 week of culture, and then formed heart and cotyledonary stage embryoson the same medium after 4 weeks. Somatic embryos matured and germinated in the presence of MS mediumcontaining 2.32 μM kinetin with (2.89μM) GA3. Plantlets were successfully transferred to pots containing amixture of soil and vermiculite (1:1).
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Paramageetham, C., Prasad Babu, G. & Rao, J. Somatic embryogenesis in Centella asiatica L. an important medicinal and neutraceutical plant of India. Plant Cell, Tissue and Organ Culture 79, 19–24 (2004). https://doi.org/10.1023/B:TICU.0000049427.27937.d2
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DOI: https://doi.org/10.1023/B:TICU.0000049427.27937.d2