Abstract
IN the course of investigations into the application of paper chromatography to the identification of reducing substances in urine it was found that, when ammoniacal silver nitrate was used as a developing reagent, urates, normally present in urine, produced a dark brown spot with an RF value of approximately 0·20. This spot, which generally ‘tails', tends to mask the spots produced by other reducing substances with RF values in the region of 0·20, namely, those of glucose, sorbose, arabinose and fructose.
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Partridge, S. M., Biochem. J., 42, 238, (1948).
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HORROCKS, R. Paper Partition Chromatography of Reducing Sugars with Benzidine as a Spraying Reagent. Nature 164, 444 (1949). https://doi.org/10.1038/164444a0
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DOI: https://doi.org/10.1038/164444a0
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