Abstract
A COMPLICATION often encountered in the use of the fluorescent antibody technique of Coons and Kaplan1 is the presence of non-specific staining material in the conjugates, even after exhaustive dialysis. Apart from its nuisance-value, the effect is of interest because it does not occur when fluorescein-globulin reacts with sections of various malignant tissues2,3.
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References
Coons, A. H., and Kaplan, M. H., J. Exp. Med., 91, 1 (1950).
Hughes, P. E., Louis, C. J., Dineen, J. K., and Spector, W. C., Nature, 180, 289 (1957).
Louis, C. J., Australasian Ann. Med., 6, 277 (1957).
Oudin, J., “Methods in Medical Research”, 5, pp. 340–359 (Year Book Publishers, Chicago, 1952).
Raymond, S., Proc. Soc. Exp. Biol. Med. (N.Y.), 81, 278 (1952).
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CURTAIN, C. Electrophoresis of Fluorescent Antibody. Nature 182, 1305–1306 (1958). https://doi.org/10.1038/1821305a0
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DOI: https://doi.org/10.1038/1821305a0
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