Abstract
MANY parasitic nematodes have a tendency to coil considerably when placed in water or cold-fixing fluids such as alcohol or formalin. Ideally, in order to facilitate the subsequent microscopic examination, nematodes should die in an extended position. This is best achieved by the use of 70 per cent alcohol or Looss' fluid (9 parts 70 per cent alcohol and 1 part glycerol), near boiling temperature, in which nematodes uncoil and become fixed rapidly. However, hot alcohol is rarely available during field work, and a simple alternative method for killing and fixing nematodes in an extended position is likely to be desired by other workers.
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References
Lee, B., The Microtomist's Vade Mecum, edit. by Gatenby, J. B., and Beams, H. W., eleventh ed. (London, 1950).
Romeis, B., Mikroskopische Technik, fifteenth ed. (Munich, 1948).
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BERLAND, B. Uue of Glacial Acetic Acid for killing Parasitic Nematodes for Collection Purposes. Nature 191, 1320–1321 (1961). https://doi.org/10.1038/1911320a0
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DOI: https://doi.org/10.1038/1911320a0
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