Abstract
A SIMPLE method has been developed whereby micrurgy on living cells such as nuclear transplantation and cytoplasmic injection in amoebae can be performed with greater ease and rapidity. The present method differs from those used currently1–4 in that the need for a micro-hook to hold cells and the use of mineral oil (liquid paraffin) to prevent the evaporation of culture medium are eliminated. This is done by placing the cells on an agar surface. When the excess medium surrounding the cells is withdrawn, they remain firmly attached during operations. The agar gel apparently replaces the medium that evaporates, and there is no damage to the cells during the period required for micrurgy. Thus, for example, nuclei are transplanted between amoebae in open drops with a micro-probe alone.
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Jeon, K. W., Exp. Cell Res., 46, 609 (1967).
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JEON, K., LORCH, I. New Simple Method of Micrurgy on Living Cells. Nature 217, 463 (1968). https://doi.org/10.1038/217463a0
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DOI: https://doi.org/10.1038/217463a0
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