Abstract
PREVIOUS work has demonstrated that the initiation of protein synthesis requires messenger RNA (mRNA)1–3, formyl-methionyl-tRNA (F-met-tRNA)4–6, ribosomes, GPT7–9, and at least three protein initiation factors10–12. Native ribosomal subunits have been shown to contain the initiation factors, and such subunits are extremely active in the initiation of protein synthesis13.
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References
Eisenstadt, J. M., and Brawerman, G., Biochemistry, 5, 2777 (1966).
Stanley, jun., W. J., Salas, M., Wahba, A. J., and Ochoa, S., Proc. US Nat. Acad. Sci., 56, 290 (1966).
Revel, M., and Gros, F., Biochem. Biophys. Res. Commun., 25, 124 (1966).
Marker, K. A., and Sanger, F., J. Mol. Biol., 8, 835 (1964).
Clark, B. F. C., and Marker, K. A., J. Mol. Biol., 17, 394 (1966).
Adams, J. M., and Capecchi, M. R., Proc. US Nat. Acad. Sci., 55, 147 (1966).
Anderson, J. S., Dahlberg, J. E., Bretscher, M. S., Revel, M., and Clark, B. F. C., Nature, 216, 1072 (1967).
Mukudan, M. A., Hershey, J. W. B., Dewey, K. F., and Thach, R., Nature, 217, 1013 (1968).
Kondo, M., Eggertsson, G., Eisenstadt, J. M., and Lengyel, P., Nature, 220, 368 (1968).
Revel, M., Herzberg, M., Becarevic, A., and Gros, F., J. Mol. Biol., 33, 231 (1968).
Iwasaki, K., Sabol, S., Miller, M. J., Wahba, A., and Ochoa, S., Fed. Proc., 27, 398 (1968).
Maitra, V., and Dubnoff, J., Fed. Proc., 27, 398 (1968).
Eisenstadt, J. M., and Brawerman, G., Proc. US Nat. Acad. Sci., 58, 1560 (1967).
Brawerman, G., and Eisenstadt, J. M., Biochemistry, 5, 2784 (1966).
Stanley, jun., W. M., and Wahba, A. J., Methods in Enzymology (edit. by Grossman, L., and Moldave, K.), 12, 524 (Academic Press, 1967).
Mans, R. J., and Novelli, G. D., Arch. Biochim. Biophys., 94, 48 (1961).
Kaempfer, R. O. R., Meselson, M., and Raskas, H. J., J. Mol. Biol., 31, 277 (1968).
Mangiarotti, G., and Schlessinger, D., J. Mol. Biol., 20, 123 (1966).
Nomura, M., and Lowry, C. V., Proc. US Nat. Acad. Sci., 58, 946 (1967).
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PARENTI-ROSINA, R., EISENSTADT, A. & EISENSTADT, J. Isolation of Protein Initiation Factors from 30S Ribosomal Subunits. Nature 221, 363–365 (1969). https://doi.org/10.1038/221363a0
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DOI: https://doi.org/10.1038/221363a0
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